Universidade de São Paulo, Department of Obstetrics and Gynecology, Ribeirão Preto/SP, Brazil.
Clinics (Sao Paulo). 2011;66(12):2093-7. doi: 10.1590/s1807-59322011001200015.
To determine the effect of storage duration on cryopreserved ovarian tissue using fresh and frozen-thawed samples.
Seventeen fertile patients underwent an ovarian biopsy during elective laparoscopic tubal ligation. The tissue sample was divided into three parts: one part was processed fresh (FG), and two were slowly frozen, cryopreserved for 30 (G30) or 180 days (G180), thawed and analyzed. Follicular density, follicular viability, and steroidogenic capacity were assessed.
We observed no differences between the groups in follicular density, which was assessed in hematoxylin and eosin-stained tissue sections. A heterogeneous follicular distribution was observed in the parenchyma, with a mean density of 361.3±255.4, 454.9±676.3, and 296.8±269.0 follicles/mm(3) for FG, G30 and G180, respectively (p = 0.46). Follicular viability was greater in FG (93.4%) when compared with the cryopreserved tissues (70.8% for G30 (p<0.001) and 78.4% for G180 (p<0.001)), with no difference in viability between the frozen samples (p>0.05). The steroidogenic capacity of the tissue was not significantly reduced following cryopreservation.
The slow freezing procedures used for ovarian cryopreservation are capable of preserving follicular viability and maintaining the steroidogenic capacity of the tissue despite a roughly 30% decrease in follicular viability. Furthermore, short-term storage of ovarian tissue does not appear to compromise follicle integrity.
通过新鲜和冷冻-解冻样本，确定储存时间对冷冻卵巢组织的影响。
17 名有生育能力的患者在择期腹腔镜输卵管结扎术中进行卵巢活检。组织样本分为三部分：一部分新鲜处理（FG），两部分缓慢冷冻，分别冷冻保存 30（G30）或 180 天（G180），解冻并分析。评估卵泡密度、卵泡活力和甾体生成能力。
我们观察到，在苏木精和伊红染色组织切片评估的卵泡密度方面，各组之间没有差异。实质内观察到卵泡分布不均匀，平均密度分别为 FG 组 361.3±255.4、G30 组 454.9±676.3 和 G180 组 296.8±269.0 个/平方毫米（p = 0.46）。FG 组（93.4%）的卵泡活力大于冷冻保存组织（G30 组为 70.8%（p<0.001），G180 组为 78.4%（p<0.001）），冷冻样本之间的活力无差异（p>0.05）。冷冻保存后，组织的甾体生成能力没有明显降低。
尽管卵泡活力降低了约 30%，但卵巢冷冻保存中使用的缓慢冷冻程序能够保存卵泡活力并维持组织的甾体生成能力。此外，卵巢组织的短期储存似乎不会损害卵泡的完整性。
