在非附着条件下进行体外卵泡生长和降低 FSH 水平会降低卵丘细胞中的 LHCGR 表达，并促进卵母细胞的发育能力。
In vitro follicle growth under non-attachment conditions and decreased FSH levels reduces Lhcgr expression in cumulus cells and promotes oocyte developmental competence.
机构信息
Follicle Biology Laboratory (FOBI), Vrije Universiteit Brussel, Laarbeeklaan, Brussels, Belgium.
出版信息
J Assist Reprod Genet. 2012 Feb;29(2):141-52. doi: 10.1007/s10815-011-9690-x. Epub 2011 Dec 22.
Abstract
PURPOSE
The in-vitro environment influences oocyte competence and gene expression in cumulus cells and oocytes. Effects of culturing under non-attachment conditions and varying follicle exposure to FSH were investigated at the mRNA level and on oocyte developmental capacity.
METHODS
Quantitative PCR analysis of Gdf9, Mater, Nmp2 (in oocytes), Lhcgr and Amh (in cumulus cells), and oocyte developmental competence after in vitro follicle culture were evaluated.
RESULTS
Follicle survival (98.7%) and polar body rate (94%) were similar for all conditions. Estradiol and progesterone production were significantly lower in non-attachment follicles (10-fold and 3-fold, respectively). Under non-attachment conditions, a higher two-cell rate (69.9%) and total blastocyst yield (48.5%) were obtained and, by decreasing FSH levels during culture, Lhcgr transcripts were significantly reduced to levels similar to in-vivo. Levels of oocyte-specific transcripts were not significantly influenced by in-vitro conditions.
CONCLUSION
Non-attachment conditions influence follicle steroid secretory capacity and, together with dynamic FSH doses, positively influence cumulus cell gene expression and oocyte developmental competence.
摘要
目的
体外环境会影响卵母细胞和卵丘细胞的能力和基因表达。本研究旨在从 mRNA 水平和卵母细胞发育能力方面，研究非附着条件下培养和卵泡暴露于不同浓度卵泡刺激素（FSH）对卵母细胞的影响。
方法
采用实时定量 PCR 分析 Gdf9、Mater、Nmp2（卵母细胞）、Lhcgr 和 Amh（卵丘细胞）的表达水平，并评估体外卵泡培养后卵母细胞的发育能力。
结果
所有条件下的卵泡存活率（98.7%）和极体率（94%）相似。非附着卵泡中的雌二醇和孕酮的产生明显降低（分别为 10 倍和 3 倍）。在非附着条件下，获得了更高的二细胞率（69.9%）和总囊胚产量（48.5%），并且通过降低培养过程中的 FSH 水平，Lhcgr 转录本的水平显著降低，与体内水平相似。卵母细胞特异性转录本的水平不受体外条件的显著影响。
结论
非附着条件会影响卵泡的类固醇分泌能力，并且与动态 FSH 剂量一起，可正向影响卵丘细胞基因表达和卵母细胞的发育能力。
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