Osteo-Articular and Dental Tissue Engineering Laboratory LIOAD, Department of Periodontology, School of Dental Surgery, INSERM U791, Nantes, France.
J Mater Sci Mater Med. 2012 Mar;23(3):797-803. doi: 10.1007/s10856-011-4534-x. Epub 2011 Dec 22.
Resorption of synthetic bone substitute materials is essential for the integration of these materials into the natural bone remodeling process. Osteoclast behavior in the presence of calcium phosphate bioceramics (CaPB) is partially understood, and a better understanding of the underlying mechanisms is expected to facilitate the development of new synthetic bone substitutes to improve bone regeneration. In the present study, our aim was to investigate osteoclastic resorption of various synthetic CaPB. We used neonatal total rabbit bone cells to generate osteoclasts. Osteoclast-generated resorption on dentine and multiple CaPB was investigated by quantifying the surface resorbed and measuring tartrate resistant acid phosphatase (TRAP) enzyme activity. In this study, we observed that osteoclastic cells responded in a different way to each substrate. Both dentine and CaPB were resorbed but the quantitative results for the surface resorbed and TRAP activity showed a specific response to each substrate and that increased mineral density seemed to inhibit osteoclast activity.
合成骨替代材料的吸收对于这些材料与自然骨重塑过程的整合至关重要。部分理解了破骨细胞在磷酸钙生物陶瓷（CaPB）存在下的行为，预计对潜在机制的更好理解将有助于开发新的合成骨替代物以改善骨再生。在本研究中，我们的目的是研究各种合成 CaPB 的破骨细胞吸收。我们使用新生的全兔骨细胞生成破骨细胞。通过定量表面吸收和测量抗酒石酸酸性磷酸酶（TRAP）酶活性来研究牙本质和多种 CaPB 上的破骨细胞生成的吸收。在这项研究中，我们观察到破骨细胞对每种底物的反应方式不同。牙本质和 CaPB 都被吸收，但表面吸收和 TRAP 活性的定量结果表明对每种底物都有特定的反应，并且矿物质密度的增加似乎抑制了破骨细胞的活性。
