Ohno S, Luka J, Klein G, Daniel M D
Proc Natl Acad Sci U S A. 1979 Apr;76(4):2042-6. doi: 10.1073/pnas.76.4.2042.
In vitro binding of a Herpesvirus ateles (HVA)-associated soluble antigen to amphibian erythrocyte nuclei was demonstrated by the acid-fixed nuclear binding technique in combination with anticomplement immunofluorescence. Incubation of concentrated salt-extracted soluble antigens derived from HVA-carrying marmoset lines with methanol/acetic acid-fixed erythrocytes of frogs and salamanders resulted in a brilliant nuclear fluorescence after exposure to a live virus-boostered, anti-HVS-positive squirrel monkey serum. Anti-HVS-negative sera did not stain. The activity of the positive serum could be abosrbed completely with extracts of HVA-carrying cells but not with Epstein-Barr virus-carrying or Herpesvirus papio-carrying cells. The HVA-associated antigen was also present in lytically HVA-infected marmoset kidney cells.
通过酸固定核结合技术结合抗补体免疫荧光法，证明了一种与蛛猴疱疹病毒（HVA）相关的可溶性抗原在体外与两栖类红细胞核的结合。将来自携带HVA的狨猴系的浓缩盐提取可溶性抗原与青蛙和蝾螈的甲醇/乙酸固定红细胞一起孵育，在用活病毒增强的抗HVS阳性松鼠猴血清处理后，产生了明亮的核荧光。抗HVS阴性血清未染色。阳性血清的活性可以被携带HVA的细胞提取物完全吸收，但不能被携带爱泼斯坦-巴尔病毒或携带狒狒疱疹病毒的细胞提取物吸收。HVA相关抗原也存在于被HVA裂解感染的狨猴肾细胞中。
