Falk L, Wolfe L, Deinhardt F
IARC Sci Publ (1971). 1975(11 Pt 1):379-84.
Continuous lymphoblastoid cell cultures were established after transformation invitro with HVA of marmoset splenic or circulating lymphocytes. Transformation was achieved by co-cultivating lymphocytes with lethally X-irradiated, HVA-carrying cells (derived originally from tumour cells of a marmoset experimentally infected with HVA) or by infecting marmoset lymphocytes with cell-free virus. Tenty-eight continous cultures from 39 that underwent co-cultivation and two of four lymphocyte preparations infected with virus became transformed. Cultivation periods before transformation were in the range 17-32 days (co-cultivation) or 51-53 days (cell-free virus). HVA genome expression in transfromed cultures was demonstrated by:(1) recovery of small amounts of HVA from culture fluids; (2) ability of lymphoblasts to induce infectious centres after co-cultivation with permissive cells; and (3) observation of antigen-positive cells after staining with monospecific antiserum. Most cultures were composed of T lymphocytes: cells of 16 of 24 cultures formed rosettes with sheep erythrocytes and none of 30 possessed membrane Ig.
用绒猴脾脏或循环淋巴细胞的人猿空泡病毒（HVA）体外转化后建立了连续的淋巴母细胞系培养物。转化是通过将淋巴细胞与经致死剂量X射线照射的携带HVA的细胞（最初来源于实验感染HVA的绒猴肿瘤细胞）共同培养，或用无细胞病毒感染绒猴淋巴细胞来实现的。在39个进行共同培养的样本中有28个连续培养物，以及在4个用病毒感染的淋巴细胞制备物中有2个发生了转化。转化前的培养期为17 - 32天（共同培养）或51 - 53天（无细胞病毒）。在转化的培养物中HVA基因组表达通过以下方式得以证实：（1）从培养液中回收少量HVA；（2）淋巴母细胞与允许性细胞共同培养后诱导感染中心的能力；以及（3）用单特异性抗血清染色后观察到抗原阳性细胞。大多数培养物由T淋巴细胞组成：24个培养物中有16个的细胞与绵羊红细胞形成玫瑰花结，30个培养物中无一具有膜免疫球蛋白。
