Falk L, Johnson D, Deinhardt F
Int J Cancer. 1978 May 15;21(5):652-7. doi: 10.1002/ijc.2910210517.
Circulating lymphocytes from three species of marmoset monkeys were transformed in vitro with Herpesvirus ateles (HVA) either by co-cultivation with lethally X-irradiated HVA-producing lymphoblastoid cells or by infection with cell-free virus: 42 transformed lymphocyte cultures were obtained from 72 transformation attempts. Attempts to transform squirrel monkey lymphocytes were unsuccessful in 29 attempts. Association of HVA with each transformed culture was demonstrated by staining of antigen-positive cells in indirect fluorescent antibody (FA) tests and recovery of HVA after co-cultivation of the transformed cells with permissive monolayer cells. Cells of most transformed cultures possessed T lymphocyte properties: i.e. formation of E rosettes, reactivity with a specific anti-marmoset T lymphocyte serum and lack of surface Ig.
来自三种狨猴的循环淋巴细胞在体外通过与经致死性X射线照射的产生HVA的淋巴母细胞样细胞共培养或用无细胞病毒感染，被阿氏疱疹病毒（HVA）转化：在72次转化尝试中获得了42个转化淋巴细胞培养物。在29次尝试中，转化松鼠猴淋巴细胞未成功。通过间接荧光抗体（FA）试验中抗原阳性细胞的染色以及将转化细胞与允许性单层细胞共培养后回收HVA，证明了HVA与每个转化培养物的关联。大多数转化培养物的细胞具有T淋巴细胞特性：即形成E玫瑰花结、与特异性抗狨猴T淋巴细胞血清反应以及缺乏表面免疫球蛋白。
