Proteomics and Molecular Cell Physiology Lab, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore 641046, TN, India.
Phytomedicine. 2012 Feb 15;19(3-4):231-5. doi: 10.1016/j.phymed.2011.11.006. Epub 2011 Dec 20.
The anti-influenza A/HK (H3N2) virus activity of β-santalol was evaluated in MDCK cells and investigated the effect of β-santalol on synthesis of viral mRNAs. β-Santalol was investigated for its antiviral activity against influenza A/HK (H3N2) virus using a cytopathic effect (CPE) reduction method. β-Santalol exhibited anti-influenza A/HK (H3N2) virus activity of 86% with no cytotoxicity at the concentration of 100 μg/ml reducing the formation of a visible CPE. Oseltamivir also showed moderate antiviral activity of about 83% against influenza A/HK (H3N2) virus at the concentration of 100 μg/ml. Furthermore, the mechanism of anti-influenza virus action in the inhibition of viral mRNA synthesis was analyzed by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR), and the data indicated an inhibitory effect in late viral RNA synthesis compared with oseltamivir in the presence of 100 μg/ml of β-santalol. β-Santalol should be further studied for therapeutic and prophylactic potential especially for influenza epidemics and pandemics.
β-檀香醇在 MDCK 细胞中抗甲型流感 H3N2 病毒的活性，并研究了β-檀香醇对病毒 mRNA 合成的影响。采用细胞病变效应（CPE）减少法研究β-檀香醇对甲型流感 H3N2 病毒的抗病毒活性。β-檀香醇在 100μg/ml 的浓度下对流感 A/HK（H3N2）病毒表现出 86%的抗流感 A/HK（H3N2）病毒活性，且无细胞毒性，可减少可见 CPE 的形成。奥司他韦在 100μg/ml 的浓度下对甲型流感 H3N2 病毒也表现出约 83%的中等抗病毒活性。此外，通过逆转录-聚合酶链反应（RT-PCR）分析了抑制病毒 mRNA 合成的抗流感病毒作用机制，与 100μg/ml 的奥司他韦相比，数据表明β-檀香醇在存在时对晚期病毒 RNA 合成具有抑制作用。β-檀香醇应进一步研究其治疗和预防潜力，特别是在流感流行和大流行期间。
