Pichetpongtorn Piriyakorn, Ruangdachsuwan Sasiporn, Churod Theeraporn, Komaikul Jukrapun, Masrinoul Promsin, Yusakul Gorawit, Kitisripanya Tharita
Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
Center for Vaccine Development, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.
Heliyon. 2024 Nov 20;10(23):e40569. doi: 10.1016/j.heliyon.2024.e40569. eCollection 2024 Dec 15.
Plants belonging to the genus have shown promise in exerting antiviral activity. In this study, sp. "Khamin Oi" (CKO), a traditional Thai medicinal herb listed in Thai herbal pharmacopoeia, was subjected to extraction using a variety of solvents. Consequently, LC-MS/MS and GC-MS/MS analyses were conducted to assess the phytochemical profiles of all the crude extracts and identify the compounds potentially responsible for their antiviral effects. The antiviral effects of these extracts against the human coronavirus OC43 (HCoV-OC43) were also investigated. An in-cell ELISA was used to investigate the anti-HCoV-OC43 replication activity of the crude rhizome extracts of CKO. Among the extracts, the crude hexane extract of CKO exhibited the lowest IC (3.62 μg/mL), with a high selectivity index (SI) of 31.90, despite having the lowest curcuminoid content. While the methanolic extract of L., known for its high curcuminoid content among species, showed an IC of 14.83 μg/mL with an SI of 2.80. Further and investigations of CKO crude extract are necessary to understand its potential anti-HCoV-OC43 properties.
该属植物在发挥抗病毒活性方面已显示出前景。在本研究中，泰国草药药典中列出的传统泰国药草“Khamin Oi”（CKO），使用多种溶剂进行提取。因此，进行了液相色谱-串联质谱（LC-MS/MS）和气相色谱-串联质谱（GC-MS/MS）分析，以评估所有粗提物的植物化学特征，并鉴定可能对其抗病毒作用负责的化合物。还研究了这些提取物对人冠状病毒OC43（HCoV-OC43）的抗病毒作用。采用细胞内酶联免疫吸附测定（ELISA）来研究CKO根茎粗提物的抗HCoV-OC43复制活性。在这些提取物中，CKO的粗己烷提取物表现出最低的半数抑制浓度（IC，3.62μg/mL），尽管姜黄素类含量最低，但具有31.90的高选择性指数（SI）。而在该属物种中以高姜黄素类含量著称的姜黄甲醇提取物，其半数抑制浓度为14.83μg/mL，选择性指数为2.80。有必要对CKO粗提物进行进一步的研究和调查，以了解其潜在的抗HCoV-OC43特性。
