Genome Research Center, NODAI Research Institute, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
DNA Res. 2012;19(1):67-79. doi: 10.1093/dnares/dsr042. Epub 2011 Dec 22.
The cyanobacterium, Synechocystis sp. PCC 6803, was the first photosynthetic organism whose genome sequence was determined in 1996 (Kazusa strain). It thus plays an important role in basic research on the mechanism, evolution, and molecular genetics of the photosynthetic machinery. There are many substrains or laboratory strains derived from the original Berkeley strain including glucose-tolerant (GT) strains. To establish reliable genomic sequence data of this cyanobacterium, we performed resequencing of the genomes of three substrains (GT-I, PCC-P, and PCC-N) and compared the data obtained with those of the original Kazusa strain stored in the public database. We found that each substrain has sequence differences some of which are likely to reflect specific mutations that may contribute to its altered phenotype. Our resequence data of the PCC substrains along with the proposed corrections/refinements of the sequence data for the Kazusa strain and its derivatives are expected to contribute to investigations of the evolutionary events in the photosynthetic and related systems that have occurred in Synechocystis as well as in other cyanobacteria.
集胞藻 PCC 6803 是第一个被测定其基因组序列的蓝藻（1996 年，卡祖萨株），因此在光合作用机制、进化和分子遗传学的基础研究中扮演着重要的角色。从最初的伯克利株衍生出了许多亚株或实验室株，包括耐葡萄糖株。为了建立该蓝藻可靠的基因组序列数据，我们对三个亚株（GT-I、PCC-P 和 PCC-N）的基因组进行了重测序，并将获得的数据与存储在公共数据库中的原始卡祖萨株的数据进行了比较。我们发现每个亚株都存在序列差异，其中一些可能反映了特定的突变，这些突变可能导致其表型发生改变。我们对 PCC 亚株的重测序数据，以及对卡祖萨株及其衍生株序列数据的修正/细化，有望促进对集胞藻以及其他蓝藻中发生的光合作用和相关系统进化事件的研究。
