Ouédraogo Moustapha, Lamien-Sanou Assita, Ramdé Norbert, Ouédraogo Aimé S, Ouédraogo Moussa, Zongo Salifou P, Goumbri Olga, Duez Pierre, Guissou Pierre I
Laboratory of Pharmacology and Toxicology, Health Sciences Faculty, University of Ouagadougou, 03 BP 7021 Ouagadougou, Burkina Faso.
Exp Toxicol Pathol. 2013 Mar;65(3):335-9. doi: 10.1016/j.etp.2011.11.006. Epub 2011 Dec 23.
Oxidative stress due to abnormal production of reactive oxygen species has been implicated in the nephrotoxicity induced by gentamicin. The nephroprotective effect of aqueous-ethanolic extract of Moringa oleifera leaves (150 and 300 mg/kg) was evaluated against gentamicin-induced (80 mg/kg) renal injury in rabbits. Serum urea and creatinine levels were evaluated as the markers of renal nephrotoxicity. At the end of the experiment, the kidneys of rabbits were excised for histological examinations and determination of lipid peroxidation levels. Serum urea and creatinine levels were reduced in the M. oleifera (150 and 300 mg/kg) plus gentamicin treated groups. On histological examinations, kidney of intoxicated rabbits groups which received M. oleifera extract showed reparative tendencies. A highly significant (p < 0.01) elevation was observed in lipid peroxidation (LPO) level in the kidneys of gentamicin-intoxicated rabbits whereas combined treatment of M. oleifera and gentamicin group showed a highly significant (p < 0.01) depletion in LPO. The present study indicates that aqueous-ethanolic extract of M. oleifera leaves attenuates renal injury in rabbits treated with gentamicin, possibly by inhibiting lipid peroxidation.
活性氧异常产生所导致的氧化应激与庆大霉素引起的肾毒性有关。评估了辣木叶水乙醇提取物（150和300毫克/千克）对庆大霉素（80毫克/千克）诱导的家兔肾损伤的肾保护作用。将血清尿素和肌酐水平作为肾毒性的标志物进行评估。实验结束时，切除家兔的肾脏进行组织学检查并测定脂质过氧化水平。在辣木（150和300毫克/千克）加庆大霉素治疗组中，血清尿素和肌酐水平降低。组织学检查显示，接受辣木提取物的中毒家兔组的肾脏有修复趋势。庆大霉素中毒家兔肾脏中的脂质过氧化（LPO）水平显著升高（p < 0.01），而辣木与庆大霉素联合治疗组的LPO水平则显著降低（p < 0.01）。本研究表明，辣木叶水乙醇提取物可能通过抑制脂质过氧化减轻庆大霉素治疗家兔的肾损伤。
