微米分辨率的多蛋白微接触印刷术。
Multiprotein microcontact printing with micrometer resolution.
机构信息
Department of Bioengineering, University of Utah, Salt Lake City, Utah 84112, USA.
出版信息
Langmuir. 2012 Jan 31;28(4):2238-43. doi: 10.1021/la2039202. Epub 2012 Jan 9.
Abstract
Depositing multiple proteins on the same substrate in positions similar to the natural cellular environment is essential to tissue engineering and regenerative medicine. In this study, the development and verification of a multiprotein microcontact printing (μCP) technique is described. It is shown that patterns of multiple proteins can be created by the sequential printing of proteins with micrometer precision in registration using an inverted microscope. Soft polymeric stamps were fabricated and mounted on a microscope stage while the substrate to be stamped was placed on a microscope objective and kept at its focal distance. This geometry allowed for visualization of patterns during the multiple stamping events and facilitated the alignment of multiple stamped patterns. Astrocytes were cultured over stamped lane patterns and were seen to interact and align with the underlying protein patterns.
摘要
在同一基底上沉积多个位置类似于自然细胞环境的蛋白质对于组织工程和再生医学至关重要。本研究描述了一种多蛋白质微接触印刷（μCP）技术的开发和验证。结果表明，可以通过使用倒置显微镜以微米级精度在注册中顺序打印蛋白质来创建多种蛋白质的图案。软聚合物印章被制造并安装在显微镜台上，而要打印的基底被放置在显微镜物镜上并保持在其焦距处。这种几何形状允许在多次打印事件期间观察图案，并便于对准多个打印图案。星形胶质细胞在打印的车道图案上培养，并被观察到与底层蛋白质图案相互作用和对齐。
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