Arredondo Miguel, Hackett John, de Bethencourt Fermín R, Treviño Ana, Escudero Domingo, Collado Antonio, Qiu Xiaoxing, Swanson Priscilla, Soriano Vincent, de Mendoza Carmen
Infectious Diseases Department, Hospital Carlos III, Madrid, Spain.
AIDS Res Hum Retroviruses. 2012 Sep;28(9):1089-94. doi: 10.1089/AID.2011.0149. Epub 2012 Feb 21.
Human infection with the xenotropic murine leukemia virus-related virus (XMRV) has been associated controversially with prostate cancer and chronic fatigue syndrome. Information is lacking about the mechanisms of transmission and potential risk groups for XMRV infection. Plasma and peripheral blood mononuclear cells (PBMCs) from individuals with retroviral infections, chronic viral hepatitis, autoimmune diseases, prostate cancer, chronic fatigue syndrome, and blood donors were tested for XMRV markers. Antibodies to XMRV proteins p15E and gp70 were examined using research assays. DNA extracted from PBMCs was tested for the presence of XMRV gag and env sequences. A total of 1103 specimens belonging to individuals with chronic fatigue syndrome and/or fibromyalgia (437), prostate cancer (69), HIV-1 (149), HTLV-1/2 (31), chronic hepatitis B (81), chronic hepatitis C (72), autoimmune diseases (18), and blood donors (246) were examined. Overall, three samples (0.3%) were p15E seroreactive (two HTLV-1 and one HCV patient). Another 15 (1.4%) were gp70 seroreactive (six chronic fatigue syndrome-fibromyalgia, four blood donors, two HIV-1, one prostate cancer, one HBV, and one HCV). Four specimens were initially positive for XMRV gag sequences, but none could be confirmed by repeated testing. In summary, no evidence of XMRV infection was found in populations with retroviral and viral hepatitis infections in Spain. Likewise, XMRV was not recognized in patients with autoimmune diseases, chronic fatigue syndrome-fibromyalgia, prostate cancer, or healthy blood donors.
人类感染异嗜性小鼠白血病病毒相关病毒（XMRV）与前列腺癌和慢性疲劳综合征存在争议性关联。目前缺乏关于XMRV传播机制及潜在风险人群的信息。对患有逆转录病毒感染、慢性病毒性肝炎、自身免疫性疾病、前列腺癌、慢性疲劳综合征的个体以及献血者的血浆和外周血单个核细胞（PBMC）进行了XMRV标志物检测。使用研究性检测方法检测针对XMRV蛋白p15E和gp70的抗体。检测从PBMC中提取的DNA是否存在XMRV gag和env序列。共检测了1103份标本，这些标本分别来自患有慢性疲劳综合征和/或纤维肌痛（437例）、前列腺癌（69例）、HIV-1（149例）、HTLV-1/2（31例）、慢性乙型肝炎（81例）、慢性丙型肝炎（72例）、自身免疫性疾病（18例）的个体以及献血者（246例）。总体而言，三份样本（0.3%）p15E血清反应阳性（两名HTLV-1感染者和一名HCV患者）。另外15份样本（1.4%）gp70血清反应阳性（6例慢性疲劳综合征-纤维肌痛患者、4名献血者、2例HIV-1感染者、1例前列腺癌患者、1例HBV感染者和1例HCV感染者）。4份标本最初XMRV gag序列呈阳性，但经重复检测均未得到确认。总之，在西班牙患有逆转录病毒感染和病毒性肝炎感染的人群中未发现XMRV感染的证据。同样，在患有自身免疫性疾病、慢性疲劳综合征-纤维肌痛、前列腺癌的患者或健康献血者中也未检测到XMRV。
