Department of Chemotherapy, Zhejiang Cancer Hospital, Hangzhou, Zhejiang, 310022, China.
Mol Biol Rep. 2012 May;39(5):5459-64. doi: 10.1007/s11033-011-1346-5. Epub 2011 Dec 30.
Anti-tumor activity and mechanism of matrine is evaluated and investigated. MTT assay showed that the matrine was able to inhibit gastric cancer cell line MNK45 in a dose-dependent manner. The concentration required for 50% inhibition (IC50) was found to be 540 μg/ml. This anti-tumor function was achieved through modulation of the NF-κB, XIAP, CIAP, and p-ERK proteins expression in cell line MNK45. By western blot analysis, we found that expression of NF-κB, XIAP, CIAP, and p-ERK proteins in cell line MNK45 would vary with varying concentration of matrine. These protein interactions possibly play a pivotal role in the regulation of apoptosis, for which further detailed analyzes are need. These results overall indicate that matrine can be used as an effective anti-tumor agent in therapy of gastric cancer.
苦参碱的抗肿瘤活性及机制研究。MTT 法检测苦参碱对胃癌 MNK45 细胞的抑制作用呈浓度依赖性，苦参碱对 MNK45 细胞的半数抑制浓度（IC50）为 540μg/ml。苦参碱通过调节 NF-κB、XIAP、CIAP 和 p-ERK 蛋白的表达来发挥抗肿瘤作用。Western blot 分析发现，苦参碱作用于 MNK45 细胞后，NF-κB、XIAP、CIAP 和 p-ERK 蛋白的表达随苦参碱浓度的变化而变化。这些蛋白相互作用可能在调控细胞凋亡中发挥关键作用，需要进一步的详细分析。综上所述，苦参碱可作为胃癌治疗的有效抗肿瘤药物。
