Yu Hai-Bo, Zhang Hui-Feng, Li De-Yu, Zhang Xiao, Xue Huan-Zhou, Zhao Si-Hai
Department of Hepatobiliary Surgery of Henan Provincial People's Hospital, Zhengzhou, China.
J Asian Nat Prod Res. 2011 Mar;13(3):242-50. doi: 10.1080/10286020.2010.551641.
Matrine is the major active component of the traditional Chinese medicine Sophora flavescens, but the molecular mechanisms of matrine on tumor invasion inhibition remain unclear. The aim of this study is to elucidate the effects of matrine on invasion ability of human hepatocellular carcinoma (HCC) cells, matrix metalloproteinase-9 (MMP-9), and nuclear factor (NF)-kappa B expression. The expression activity of MMP-9 was measured by reverse transcription polymerase chain reaction, Western blot, and gelatin zymography analysis. The expression of NF-kappa B was measured by the Western blot analysis. Matrine significantly inhibited MMP-9 expression of SMMC-7721 cells. NF-kappa B inhibitor PTDC induced a marked reduction in MMP-9 expression, and it suggested that NF-kappa B could play an important role in MMP-9 expression. Furthermore, matrine significantly suppressed NF-kappa B expression and the invasion of SMMC-7721 cells. Our results showed that matrine inhibited MMP-9 expression and the invasion of human HCC cells. The inhibitory effects are partly associated with the downregulation of the NF-kappa B signaling pathway.
苦参碱是传统中药苦参的主要活性成分，但苦参碱抑制肿瘤侵袭的分子机制尚不清楚。本研究旨在阐明苦参碱对人肝癌（HCC）细胞侵袭能力、基质金属蛋白酶-9（MMP-9）和核因子（NF）-κB表达的影响。通过逆转录聚合酶链反应、蛋白质免疫印迹法和明胶酶谱分析测定MMP-9的表达活性。通过蛋白质免疫印迹分析测定NF-κB的表达。苦参碱显著抑制SMMC-7721细胞中MMP-9的表达。NF-κB抑制剂PTDC显著降低MMP-9的表达，这表明NF-κB可能在MMP-9的表达中起重要作用。此外，苦参碱显著抑制NF-κB的表达以及SMMC-7721细胞的侵袭。我们的结果表明，苦参碱抑制MMP-9的表达以及人肝癌细胞的侵袭。其抑制作用部分与NF-κB信号通路的下调有关。
