Lee Seung-Min, Jeong Ji-Seon, Kwon Ha-Jeong, Hong Seon-Pyo
Department of Oriental Pharmaceutical Sciences, Kyung Hee East-West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, Seoul 130-701, South Korea.
Center for Bioanalysis, Department of Metrology for Quality of Life, Korea Research Institute of Standards and Science, 267 Gajeong-ro, Yuseong-gu, Daejeon 34113, South Korea; Department of Bio-Analytical Science, University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, South Korea.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Dec 1;1070:76-81. doi: 10.1016/j.jchromb.2017.10.046. Epub 2017 Oct 23.
Astragali Radix, the root of Astragalus membranaceus Bunge, is one of the most frequently used crude drugs in Asian medicine. We developed a quantification method for 6 components (calycosin, formononetin, astragaloside I-IV) of Astragali Radix and Hwanggi-gyeji-omul-tang (HGOT) using reverse-phase high-performance liquid chromatography coupled with integrated pulsed amperometric detection (RP-HPLC-IPAD). The plants were extracted in 80% ethanol for 2h. All target components were detected with good sensitivity using sodium hydroxide (as a post-column eluent). The limit of detection (S/N=3) and limit of quantification (S/N=10) of the target components ranged from 0.10-1.00ng and from 0.30-3.00ng, respectively. The coefficients of linear regression ranged from 0.9993-1.0000, all interday and intraday precision values were <3.64%, and the average recovery ranged from 99.00-102.97% for Astragali Radix and 97.73-102.57% for HGOT. This method exhibited good selectivity, sensitivity, and reproducibility and can be used directly without any pretreatment steps. Our method will therefore be useful as a quality control measure for Astragali Radix.
黄芪，即蒙古黄芪的根，是亚洲医学中最常用的中药材之一。我们开发了一种使用反相高效液相色谱结合积分脉冲安培检测法（RP-HPLC-IPAD）对黄芪及黄芪桂枝五物汤（HGOT）中的6种成分（毛蕊异黄酮、芒柄花素、黄芪甲苷I-IV）进行定量的方法。将植物用80%乙醇提取2小时。使用氢氧化钠（作为柱后洗脱液）对所有目标成分进行检测，灵敏度良好。目标成分的检测限（S/N=3）和定量限（S/N=10）分别为0.10 - 1.00 ng和0.30 - 3.00 ng。线性回归系数范围为0.9993 - 1.0000，所有日间和日内精密度值均<3.64%，黄芪的平均回收率为99.00 - 102.97%，HGOT的平均回收率为97.73 - 102.57%。该方法具有良好的选择性、灵敏度和重现性，无需任何预处理步骤即可直接使用。因此，我们的方法将作为黄芪质量控制的一种有用措施。
