Department of Chemical Engineering & Applied Chemistry, University of Toronto, Toronto, Ontario, Canada.
Appl Environ Microbiol. 2012 Mar;78(5):1596-600. doi: 10.1128/AEM.06511-11. Epub 2011 Dec 30.
The abundances of nine transcripts predicted to encode lignocellulose-modifying enzymes were measured over the course of Phanerochaete carnosa cultivation on four wood species. Profiles were consistent with sequential decay; transcripts encoding lignin-degrading peroxidases featured a significant substrate-dependent response. The chitin synthase gene was identified as the optimal internal reference gene for transcript quantification.
测定了 9 种预测编码木质纤维素修饰酶的转录本在 Phanerochaete carnosa 生长于四种木材上的过程中的丰度。谱型与顺序降解一致;编码木质素降解过氧化物酶的转录本表现出显著的底物依赖性反应。几丁质合酶基因被确定为转录本定量的最佳内参基因。