Aoki Katsuyuki, Murayama Kazutaka
Department of Materials Science, Toyohashi University of Technology, Tempaku-cho, Toyohashi, Japan.
Met Ions Life Sci. 2012;10:43-102. doi: 10.1007/978-94-007-2172-2_2.
Metal ions play a key role in nucleic acid structure and activity. Elucidation of the rules that govern the binding of metal ions is therefore an essential step for better understanding of the nucleic acid functions. This review is as an update to a preceding one (Metal Ions Biol. Syst., 1996, 32, 91-134), in which we offered a general view of metal ion interactions with mono-, di-, tri-, and oligonucleotides in the solid state, based on their crystal structures reported before 1994. In this chapter, we survey all the crystal structures of metal ion complexes with nucleotides involving oligonucleotides reported after 1994 and we have tried to uncover new characteristic metal bonding patterns for mononucleotides and oligonucleotides with A-RNA and A/B/Z-DNA fragments that form duplexes. We do not cover quadruplexes, duplexes with metal-mediated base-pairs, tRNAs, rRNAs in ribosome, ribozymes, and nucleic acid-drug and -protein complexes. Factors that affect metal binding to mononucleotides and oligonucleotide duplexes are also dealt with.
金属离子在核酸结构和活性中起着关键作用。因此，阐明金属离子结合的规律是更好地理解核酸功能的重要一步。本综述是对之前一篇综述（《金属离子生物系统》，1996年，32卷，91 - 134页）的更新，在那篇综述中，我们基于1994年以前报道的晶体结构，对金属离子与固态单核苷酸、二核苷酸、三核苷酸及寡核苷酸的相互作用进行了概述。在本章中，我们研究了1994年以后报道的金属离子与包含寡核苷酸的核苷酸形成的配合物的所有晶体结构，并试图揭示单核苷酸和具有形成双链体的A - RNA及A/B/Z - DNA片段的寡核苷酸新的特征性金属键合模式。我们不涉及四链体、具有金属介导碱基对的双链体、转运RNA、核糖体中的核糖体RNA、核酶以及核酸 - 药物和 - 蛋白质复合物。还讨论了影响金属与单核苷酸和寡核苷酸双链体结合的因素。
