Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University in Bratislava, Bratislava, Slovak Republic.
FEMS Microbiol Lett. 2012 Mar;328(2):138-43. doi: 10.1111/j.1574-6968.2011.02491.x. Epub 2012 Jan 10.
CTBT (7-chlorotetrazolo[5,1-c]benzo[1,2,4]triazine) causes intracellular superoxide production and oxidative stress and enhances the susceptibility of Saccharomyces cerevisiae, Candida albicans, and C. glabrata cells to cycloheximide, 5-fluorocytosine, and azole antimycotic drugs. Here, we demonstrate the antifungal activity of CTBT against 14 tested filamentous fungi. CTBT prevented spore germination and mycelial proliferation of Aspergillus niger and the pathogenic Aspergillus fumigatus. The action of CTBT is fungicidal. CTBT increased the formation of reactive oxygen species in fungal mycelium as detected by 2',7'-dichlorodihydrofluorescein diacetate and reduced the radial growth of colonies in a dose-dependent manner. Co-application of CTBT and itraconazole led to complete inhibition of fungal growth at dosages lower than the chemicals alone. Antifungal and chemosensitizing activities of CTBT in filamentous fungi may be useful in combination treatments of infections caused by drug-resistant fungal pathogens.
CTBT（7-氯四唑并[5,1-c]苯并[1,2,4]三嗪）可导致细胞内超氧自由基产生和氧化应激，并增强酿酒酵母、白色念珠菌和光滑念珠菌细胞对环丝氨酸、5-氟胞嘧啶和唑类抗真菌药物的敏感性。在这里，我们证明了 CTBT 对 14 种测试丝状真菌的抗真菌活性。CTBT 可预防黑曲霉和致病性烟曲霉的孢子萌发和菌丝增殖。CTBT 的作用是杀菌性的。CTBT 通过 2',7'-二氯二氢荧光素二乙酸酯检测到增加了真菌菌丝中活性氧的形成，并以剂量依赖性方式降低了菌落的径向生长。CTBT 与伊曲康唑联合应用可在低于单独使用这些化学物质的剂量下完全抑制真菌生长。CTBT 在丝状真菌中的抗真菌和化学增敏活性可能有助于治疗耐药真菌病原体引起的感染。
