Ministério da Agricultura, Pecuária e Abastecimento, Laboratório Nacional Agropecuário - LANAGRO/RS, Estrada da Ponta Grossa, 3036, CEP 91780-580, Porto Alegre, RS, Brazil.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2012;29(4):577-86. doi: 10.1080/19440049.2011.641508. Epub 2012 Jan 3.
Two bioactivity-based screening methods for antibiotic residue analysis (FAST Antimicrobial Screening Test and PremiTest) were compared, in terms of sensitivity, with a new in-house developed tube test assay using Escherichia coli. Tests were performed using antibiotic standards, spiked samples and real incurred samples. The minimum inhibitory concentration (MIC) for several antibiotics was established and compared with maximum residue levels (MRLs) in samples. The results of all evaluated tests are compared with liquid chromatography-tandem mass spectrometry multi-residue screening tests to compare parameters such as sample preparation, cost, time of analysis and confidence in results. For all tests, values of half the maximum residue limit (0.5 × MRL) were considered as a satisfactory target for a screening method. The potential and limitations of each method are discussed to indicate more rational and effective strategies for high-throughput residue monitoring and surveillance programmes. It was concluded that bioactivity-based screening methods are a useful tool, but the best compromise between minimum performance limits, cost and selectivity must be taken into account. For laboratories equipped with mass spectrometry, multi-class screening methods provide more specific responses with high sensitivity.
两种基于生物活性的抗生素残留分析筛选方法（FAST 抗菌筛选测试和 PremiTest）在灵敏度方面与使用大肠杆菌开发的内部新试管测试方法进行了比较。使用抗生素标准品、加标样品和实际发生的样品进行了测试。确定了几种抗生素的最小抑菌浓度 (MIC)，并与样品中的最大残留限量 (MRL) 进行了比较。将所有评估测试的结果与液相色谱-串联质谱多残留筛选测试进行了比较，比较了样品制备、成本、分析时间和结果可信度等参数。对于所有测试，将最大残留限量的一半 (0.5 × MRL) 值视为筛选方法的令人满意的目标。讨论了每种方法的潜力和局限性，以确定用于高通量残留监测和监测计划的更合理和有效的策略。结论是，基于生物活性的筛选方法是一种有用的工具，但必须考虑最低性能限制、成本和选择性之间的最佳折衷。对于配备质谱的实验室，多类筛选方法具有更高的灵敏度和更特异性的响应。
