Department of Clinical Methodology and Medical Surgery Technology, University of Bari, Bari, Italy.
J Biol Regul Homeost Agents. 2011 Oct-Dec;25(4):603-14.
Applications of laser therapy in biostimulation and healing injured tissues are widely described in medical literature. The present study focuses on the effects of laser irradiation on the growth rate and differentiation of human osteoblast-like cells seeded on titanium or zirconia surfaces. Cells were laser irradiated with low therapeutical doses at different intervals and the effects of irradiation were evaluated at each time-point. After 3 hours lasered cells showed an enhanced mitogen activity compared to non-lasered control cells and a higher alkaline phosphatase activity, marker of bone formation. At the same time, the mRNA of RUNX2 and OSTERIX, two genes involved in osteoblast differentiation, showed a clear decrease in lasered cells. This reached the lowest value 6 to 12 hours after irradiation, after which the transcripts started to increase, indicating that the laser treatment did promote the osteogenic potential of growth-induced cells. These results indicate that Low Level Laser Treatment (LLLT) stimulates osteogenic cell proliferation.
激光疗法在生物刺激和受伤组织修复中的应用在医学文献中有广泛的描述。本研究侧重于激光照射对种植在钛或氧化锆表面的人成骨样细胞的生长速度和分化的影响。细胞以不同的时间间隔用低治疗剂量激光照射，并在每个时间点评估照射的效果。激光照射 3 小时后，与未激光照射的对照细胞相比，激光照射细胞的有丝分裂原活性增强，碱性磷酸酶活性升高，这是骨形成的标志物。同时，参与成骨细胞分化的两个基因 RUNX2 和 OSTERIX 的 mRNA 在激光照射细胞中明显减少。这种情况在照射后 6 到 12 小时达到最低值，之后转录本开始增加，表明激光处理确实促进了诱导生长细胞的成骨潜能。这些结果表明，低水平激光治疗（LLLT）刺激成骨细胞增殖。
