斑马鱼原肠胚形成过程中细胞形状和极性的分析。
Analysis of cell shape and polarity during zebrafish gastrulation.
作者信息
Weiser Douglas C, Kimelman David
机构信息
Department of Biological Sciences, University of the Pacific, Stockton, CA, USA.
出版信息
Methods Mol Biol. 2012;839:53-68. doi: 10.1007/978-1-61779-510-7_5.
Gastrulation is a complex set of cellular rearrangements that establish the overall shape of the body plan during development. In addition to being an essential and fascinating aspect of development, the cells of the gastrulating zebrafish embryo also provide an ideal in vivo system to study the interplay of cell polarity and movement in a native 3D environment. During gastrulation, zebrafish mesodermal cells undergo a series of conversions from initial non-polarized amoeboid cell movements to more mesenchymal and finally highly polarized and intercalative cell behaviors. Many of the cellular behavior changes of these cells are under the control of the RhoA pathway, which in turn is regulated by many signals, including non-canonical Wnts. The goal of this chapter is to provide researchers with the necessary protocols to examine changes in cell polarity and movement in the developing zebrafish embryo.
原肠胚形成是一系列复杂的细胞重排过程，在发育过程中确立身体蓝图的整体形态。除了是发育过程中一个至关重要且引人入胜的方面外，处于原肠胚形成阶段的斑马鱼胚胎细胞还提供了一个理想的体内系统，用于研究细胞极性与运动在天然三维环境中的相互作用。在原肠胚形成过程中，斑马鱼中胚层细胞经历了一系列转变，从最初的非极化阿米巴样细胞运动转变为更多的间充质细胞运动，最终转变为高度极化和插入性的细胞行为。这些细胞的许多行为变化受RhoA信号通路控制，而该信号通路又受包括非经典Wnt信号在内的多种信号调节。本章的目的是为研究人员提供必要的实验方案，以检测发育中的斑马鱼胚胎细胞极性和运动的变化。
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