Merabishvili Maia, Vandenheuvel Dieter, Kropinski Andrew M, Mast Jan, De Vos Daniel, Verbeken Gilbert, Noben Jean-Paul, Lavigne Rob, Vaneechoutte Mario, Pirnay Jean-Paul
Laboratory for Molecular and Cellular Technology (LabMCT), Queen Astrid Military Hospital, Brussels, Belgium; Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi, Georgia; Laboratory for Bacteriology Research (LBR), Faculty Medicine & Health Sciences, Ghent University, Ghent, Belgium.
Laboratory of Gene Technology, Faculty of Bioscience Engineering, Katholieke Universiteit Leuven, Leuven, Belgium.
PLoS One. 2014 Aug 11;9(8):e104853. doi: 10.1371/journal.pone.0104853. eCollection 2014.
Based on genotyping and host range, two newly isolated lytic bacteriophages, myovirus vB_AbaM_Acibel004 and podovirus vB_AbaP_Acibel007, active against Acinetobacter baumannii clinical strains, were selected from a new phage library for further characterization. The complete genomes of the two phages were analyzed. Both phages are characterized by broad host range and essential features of potential therapeutic phages, such as short latent period (27 and 21 min, respectively), high burst size (125 and 145, respectively), stability of activity in liquid culture and low frequency of occurrence of phage-resistant mutant bacterial cells. Genomic analysis showed that while Acibel004 represents a novel bacteriophage with resemblance to some unclassified Pseudomonas aeruginosa phages, Acibel007 belongs to the well-characterized genus of the Phikmvlikevirus. The newly isolated phages can serve as potential candidates for phage cocktails to control A. baumannii infections.
基于基因分型和宿主范围，从一个新的噬菌体文库中筛选出两种新分离的裂解性噬菌体，即针对鲍曼不动杆菌临床菌株具有活性的肌尾噬菌体vB_AbaM_Acibel004和短尾噬菌体vB_AbaP_Acibel007，用于进一步表征。对这两种噬菌体的完整基因组进行了分析。两种噬菌体均具有宿主范围广以及潜在治疗性噬菌体的基本特征，如潜伏期短（分别为27分钟和21分钟）、裂解量高（分别为125和145）、在液体培养中活性稳定以及噬菌体抗性突变细菌细胞出现频率低。基因组分析表明，Acibel004是一种新型噬菌体，与一些未分类的铜绿假单胞菌噬菌体相似，而Acibel007属于特征明确的类PhiKMV病毒属。新分离的噬菌体可作为噬菌体鸡尾酒制剂控制鲍曼不动杆菌感染的潜在候选者。
