Institute of Medicine, University of Bergen, N-5021 Bergen, Norway.
J Nutr Biochem. 2012 Nov;23(11):1384-93. doi: 10.1016/j.jnutbio.2011.08.006. Epub 2012 Jan 4.
The 3-thia fatty acid tetradecylthioacetic acid (TTA) is a synthetic modified fatty acid, which, similar with dietary fish oil (FO), influences the regulation of lipid metabolism, the inflammatory response and redox status. This study was aimed to penetrate the difference in TTA's mode of action compared to FO in a long-term experiment (50 weeks of feeding). Male Wistar rats were fed a control, high-fat (25% w/v) diet or a high-fat diet supplemented with either TTA (0.375% w/v) or FO (10% w/v) or their combination. Plasma fatty acid composition, hepatic lipids and expression of relevant genes in the liver and biomarkers of oxidative damage to protein were assessed at the end point of the experiment. Both supplements given in combination demonstrated an additive effect on the decrease in plasma cholesterol levels. The FO diet alone led to removal of plasma cholesterol and a concurrent cholesterol accumulation in liver; however, with TTA cotreatment, the hepatic cholesterol level was significantly reduced. Dietary FO supplementation led to an increased oxidative damage, as seen by biomarkers of protein oxidation and lipoxidation. Tetradecylthioacetic acid administration reduced the levels of these biomarkers confirming its protective role against lipoxidation and protein oxidative damage. Our findings explore the lipid reducing effects of TTA and FO and demonstrate that these bioactive dietary compounds might act in a different manner. The experiment confirms the antioxidant capacity of TTA, showing an improvement in FO-induced oxidative stress.
3-硫代脂肪酸十四烷基硫代乙酸（TTA）是一种合成的改性脂肪酸，与膳食鱼油（FO）类似，它影响脂质代谢、炎症反应和氧化还原状态的调节。本研究旨在通过长期实验（50 周喂养）探究 TTA 与 FO 的作用模式的差异。雄性 Wistar 大鼠分别用对照高脂（25%w/v）饮食或高脂饮食补充 TTA（0.375%w/v）或 FO（10%w/v）或两者的混合物进行喂养。在实验结束时，评估了血浆脂肪酸组成、肝脂质以及肝中相关基因的表达和蛋白质氧化损伤的生物标志物。两种补充剂联合使用表现出降低血浆胆固醇水平的相加作用。单独的 FO 饮食导致血浆胆固醇去除和同时的肝胆固醇积累；然而，用 TTA 共同处理，肝胆固醇水平显著降低。膳食 FO 补充导致蛋白质氧化和脂质过氧化生物标志物的氧化损伤增加。TTA 给药降低了这些生物标志物的水平，证实了其对脂质过氧化和蛋白质氧化损伤的保护作用。我们的研究结果探讨了 TTA 和 FO 的降血脂作用，并表明这些生物活性膳食化合物可能以不同的方式发挥作用。该实验证实了 TTA 的抗氧化能力，显示出对 FO 诱导的氧化应激的改善。
