高通量弹道注射纳米流变学测量细胞力学。
High-throughput ballistic injection nanorheology to measure cell mechanics.
机构信息
Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland, USA.
出版信息
Nat Protoc. 2012 Jan 5;7(1):155-70. doi: 10.1038/nprot.2011.436.
Abstract
High-throughput ballistic injection nanorheology is a method for the quantitative study of cell mechanics. Cell mechanics are measured by ballistic injection of submicron particles into the cytoplasm of living cells and tracking the spontaneous displacement of the particles at high spatial resolution. The trajectories of the cytoplasm-embedded particles are transformed into mean-squared displacements, which are subsequently transformed into frequency-dependent viscoelastic moduli and time-dependent creep compliance of the cytoplasm. This method allows for the study of a wide range of cellular conditions, including cells inside a 3D matrix, cell subjected to shear flows and biochemical stimuli, and cells in a live animal. Ballistic injection lasts <1 min and is followed by overnight incubation. Multiple particle tracking for one cell lasts <1 min. Forty cells can be examined in <1 h.
摘要
高通量弹道注射纳米流变学是一种定量研究细胞力学的方法。通过将亚微米颗粒弹道注射到活细胞的细胞质中，并以高空间分辨率跟踪颗粒的自发位移来测量细胞力学。将细胞质嵌入颗粒的轨迹转化为均方位移，随后将其转化为细胞质的频域粘弹性模量和时域蠕变柔量。该方法可用于研究广泛的细胞条件，包括 3D 基质中的细胞、受剪切流和生化刺激的细胞以及活体动物中的细胞。弹道注射持续时间<1 分钟，随后进行过夜孵育。一个细胞的多次颗粒追踪持续时间<1 分钟。<1 小时内可检查 40 个细胞。
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