Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia.
Eur J Pharmacol. 2012 Feb 29;677(1-3):31-8. doi: 10.1016/j.ejphar.2011.12.027. Epub 2011 Dec 27.
The current study investigates the potential toxicity of lipopolysaccharide (LPS) on the mitochondrial fraction of rat testis and the possible protective efficacy of lycopene. Adult male Wistar rats were categorized into four groups. Two groups were administered LPS (0.1mg/kg/day for 7 days i.p.); one of these groups received lycopene treatment (4 mg/kg/day by oral gavage, 24h before LPS treatment) (Group IV) and the other received LPS alone (Group III). A vehicle-treated group (Group I) and a lycopene drug control group (Group II) were also included. Sperm count and motility were significantly decreased in Group III. The testicular mitochondrial fraction of Group III showed significant increase in basal and Fe(2+)-induced lipid peroxidation, along with a significant increase in hydrogen peroxide (H(2)O(2)) level. Moreover, the activities of mitochondrial enzymic (SOD, CAT, GPx, GR and ADH) and non-enzymic (GSH and ascorbate) antioxidants levels were decreased. Group III also showed decline in the activities of TCA enzymes such as SDH, MDH and ICDH. Pretreatment with lycopene showed normal sperm parameters, lipid peroxidation, H(2)O(2) level, antioxidant defenses and TCA enzyme activities. In conclusion, this study indicates that LPS-induced oxidative stress leads to functional damages in the testicular mitochondria. Lycopene pretreatment provided a marked normalization in these parameters.
本研究旨在探讨脂多糖（LPS）对大鼠睾丸线粒体部分的潜在毒性，以及番茄红素的可能保护作用。成年雄性 Wistar 大鼠被分为四组。两组给予 LPS（0.1mg/kg/天，腹腔注射，共 7 天）；其中一组给予番茄红素治疗（4mg/kg/天，灌胃，LPS 处理前 24 小时开始）（第四组），另一组给予 LPS 单独处理（第三组）。还包括一组给予 vehicle 处理的大鼠（第一组）和一组给予番茄红素药物对照的大鼠（第二组）。第三组大鼠的精子计数和活力显著下降。第三组大鼠的睾丸线粒体部分显示出基础和 Fe(2+)-诱导的脂质过氧化显著增加，同时过氧化氢（H(2)O(2)）水平显著升高。此外，线粒体酶（SOD、CAT、GPx、GR 和 ADH）和非酶（GSH 和抗坏血酸）抗氧化剂的活性降低。第三组大鼠的 TCA 酶（如 SDH、MDH 和 ICDH）的活性也下降。番茄红素预处理显示精子参数、脂质过氧化、H(2)O(2)水平、抗氧化防御和 TCA 酶活性正常。总之，本研究表明 LPS 诱导的氧化应激导致睾丸线粒体功能损伤。番茄红素预处理使这些参数明显恢复正常。
