Department of Biological Sciences, Boise State University, Boise, ID 83725, USA.
Electrophoresis. 2012 Jan;33(2):366-9. doi: 10.1002/elps.201100335.
RNA-based applications requiring high-quality, non-degraded RNA are a foundational element of many research studies. As such, it is paramount that the integrity of experimental RNA is validated prior to cDNA synthesis or other downstream applications. In the absence of expensive equipment such as microfluidic electrophoretic devices, and as an alternative to the costly and time-consuming standard formaldehyde gel, RNA quality can be quickly analyzed by adding small amounts of commercial bleach to TAE buffer-based agarose gels prior to electrophoresis. In the presence of low concentrations of bleach, the secondary structure of RNA is denatured and potential contaminating RNases are destroyed. Because of this, the 'bleach gel' is a functional approach that addresses the need for an inexpensive and safe way to evaluate RNA integrity and will improve the ability of researchers to rapidly analyze RNA quality.
基于 RNA 的应用需要高质量、无降解的 RNA，这是许多研究的基础。因此，在进行 cDNA 合成或其他下游应用之前，验证实验 RNA 的完整性至关重要。在没有微流控电泳设备等昂贵设备的情况下，并且作为昂贵且耗时的标准甲醛凝胶的替代方法，可以在电泳前向基于 TAE 缓冲液的琼脂糖凝胶中添加少量商业漂白剂来快速分析 RNA 质量。在低浓度漂白剂的存在下，RNA 的二级结构被变性，潜在的污染 RNase 被破坏。因此，“漂白凝胶”是一种实用的方法，可满足寻找廉价且安全的评估 RNA 完整性的方法的需求，并提高研究人员快速分析 RNA 质量的能力。
