接触蛋白相关蛋白 1（Caspr1）调节α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）型谷氨酸受体的运输和突触含量。
Contactin-associated protein 1 (Caspr1) regulates the traffic and synaptic content of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors.
机构信息
CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal.
出版信息
J Biol Chem. 2012 Feb 24;287(9):6868-77. doi: 10.1074/jbc.M111.322909. Epub 2012 Jan 5.
Abstract
Glutamate receptors of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) type mediate fast excitatory synaptic transmission in the CNS. Synaptic strength is modulated by AMPA receptor binding partners, which regulate receptor synaptic targeting and functional properties. We identify Contactin-associated protein 1 (Caspr1) as an AMPA receptor interactor. Caspr1 is present in synapses and interacts with AMPA receptors in brain synaptic fractions. Coexpression of Caspr1 with GluA1 increases the amplitude of glutamate-evoked currents. Caspr1 overexpression in hippocampal neurons increases the number and size of synaptic GluA1 clusters, whereas knockdown of Caspr1 decreases the intensity of synaptic GluA1 clusters. Hence, Caspr1 is a regulator of the trafficking of AMPA receptors to synapses.
摘要
α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）型谷氨酸受体介导中枢神经系统中的快速兴奋性突触传递。突触强度受 AMPA 受体结合伴侣调节，这些伴侣调节受体的突触靶向和功能特性。我们发现接触蛋白相关蛋白 1（Caspr1）是一种 AMPA 受体相互作用蛋白。Caspr1 存在于突触中，并与脑突触部分的 AMPA 受体相互作用。Caspr1 与 GluA1 的共表达增加了谷氨酸诱导电流的幅度。Caspr1 在海马神经元中的过表达增加了突触 GluA1 簇的数量和大小，而 Caspr1 的敲低则降低了突触 GluA1 簇的强度。因此，Caspr1 是 AMPA 受体向突触转运的调节剂。
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