Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Biosens Bioelectron. 2012 Feb 15;32(1):238-43. doi: 10.1016/j.bios.2011.12.021. Epub 2011 Dec 23.
In this study, electrochemical immunoassay was introduced into the recently proposed microfluidic paper-based analytical device (μPADs). To improve the performance of electrochemical immunoassay on μPAD for point-of-care testing (POCT), a novel wax-patterned microfluidic paper-based three-dimensional electrochemical device (3D-μPED) was demonstrated based on the multi-walled carbon nanotubes (MWCNTs) modified μPAD. Using typical HRP-O-Phenylenediamine-H(2)O(2) electrochemical system, a sandwich immunoassay on this 3D-μPED for sensitive diagnosis of two tumor markers simultaneously in real clinical serum samples was developed with a linear range of 0.001-75.0 UmL(-1) for cancer antigen 125 and 0.05-50.0 ngmL(-1) for carcinoembryonic antigen. In addition, this 3D-μPED can be easily integrated and combined with the recently emerging paper electronics to further develop simple, sensitive, low-cost, disposable and portable μPAD for POCT, public health and environmental monitoring in remote regions, developing or developed countries.
在这项研究中，电化学免疫分析被引入到最近提出的微流控纸基分析装置（μPADs）中。为了提高电化学免疫分析在μPAD 上用于即时检测（POCT）的性能，基于多壁碳纳米管（MWCNTs）修饰的μPAD，展示了一种新型的蜡图案化微流控纸基三维电化学装置（3D-μPED）。使用典型的 HRP-O-苯二胺-H（2）O（2）电化学系统，在这个 3D-μPED 上进行三明治免疫分析，可以同时灵敏地诊断两种肿瘤标志物，在真实的临床血清样本中，对癌抗原 125 的线性范围为 0.001-75.0 UmL（-1），对癌胚抗原的线性范围为 0.05-50.0 ngmL（-1）。此外，这种 3D-μPED 可以很容易地与最近新兴的纸质电子集成和结合，进一步开发用于 POCT 的简单、灵敏、低成本、一次性和便携式 μPAD，用于远程地区、发展中和发达国家的公共卫生和环境监测。
