Dabbagh Sajjad Rahmani, Becher Elaina, Ghaderinezhad Fariba, Havlucu Hayati, Ozcan Oguzhan, Ozkan Mehmed, Yetisen Ali Kemal, Tasoglu Savas
Department of Biomedical Engineering, University of Connecticut, Storrs, Connecticut 06269, USA.
Department of Mechanical Engineering, University of Connecticut, Storrs, Connecticut 06269, USA.
Biomicrofluidics. 2021 Feb 4;15(1):011502. doi: 10.1063/5.0042816. eCollection 2021 Jan.
Paper-based devices have a wide range of applications in point-of-care diagnostics, environmental analysis, and food monitoring. Paper-based devices can be deployed to resource-limited countries and remote settings in developed countries. Paper-based point-of-care devices can provide access to diagnostic assays without significant user training to perform the tests accurately and timely. The market penetration of paper-based assays requires decreased device fabrication costs, including larger packing density of assays (i.e., closely packed features) and minimization of assay reagents. In this review, we discuss fabrication methods that allow for increasing packing density and generating closely packed features in paper-based devices. To ensure that the paper-based device is low-cost, advanced fabrication methods have been developed for the mass production of closely packed assays. These emerging methods will enable minimizing the volume of required samples (e.g., liquid biopsies) and reagents in paper-based microfluidic devices.
纸质设备在即时诊断、环境分析和食品监测等领域有着广泛的应用。纸质设备可以部署到资源有限的国家以及发达国家的偏远地区。基于纸的即时检测设备无需使用者接受大量培训就能准确及时地进行诊断检测。纸质检测方法要想在市场上得到广泛应用，就需要降低设备制造成本，包括提高检测的包装密度（即紧密排列的特征）并尽量减少检测试剂用量。在本综述中，我们讨论了能够提高包装密度并在纸质设备中生成紧密排列特征的制造方法。为确保纸质设备成本低廉，已开发出先进的制造方法用于大规模生产紧密排列的检测。这些新兴方法将能够减少基于纸的微流控设备中所需样品（如液体活检样本）和试剂的体积。
