Goodman Miriam B, Lindsay Theodore H, Lockery Shawn R, Richmond Janet E
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California, USA.
Methods Cell Biol. 2012;107:409-36. doi: 10.1016/B978-0-12-394620-1.00014-X.
Patch-clamp electrophysiology is a technique of choice for the biophysical analysis of the function of nerve, muscle, and synapse in Caenorhabditis elegans nematodes. Considerable technical progress has been made in C. elegans electrophysiology in the decade since the initial publication of this technique. Today, most, if not all, electrophysiological studies that can be done in larger animal preparations can also be done in C. elegans. This chapter has two main goals. The first is to present to a broad audience the many techniques available for patch-clamp analysis of neurons, muscles, and synapses in C. elegans. The second is to provide a methodological introduction to the techniques for patch clamping C. elegans neurons and body-wall muscles in vivo, including emerging methods for optogenetic stimulation coupled with postsynaptic recording. We also present samples of the cell-intrinsic and postsynaptic ionic currents that can be measured in C. elegans nerves and muscles.
膜片钳电生理学是对秀丽隐杆线虫的神经、肌肉和突触功能进行生物物理分析的首选技术。自该技术首次发表以来的十年间，秀丽隐杆线虫电生理学取得了显著的技术进步。如今，大多数（即便不是全部）能在大型动物标本上进行的电生理研究也都可以在秀丽隐杆线虫上开展。本章有两个主要目标。第一个目标是向广大读者介绍可用于对秀丽隐杆线虫的神经元、肌肉和突触进行膜片钳分析的众多技术。第二个目标是提供对在体内膜片钳记录秀丽隐杆线虫神经元和体壁肌肉技术的方法学介绍，包括用于光遗传学刺激与突触后记录相结合的新兴方法。我们还展示了可在秀丽隐杆线虫神经和肌肉中测量的细胞内在和突触后离子电流样本。
