Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Japan.
Infect Genet Evol. 2012 Mar;12(2):471-7. doi: 10.1016/j.meegid.2011.12.012. Epub 2011 Dec 27.
The group A rotavirus (RVA) P[19] is a rare P-genotype of the RVA VP4 gene, reported so far in humans and pigs. Whole genomic analyses of P[19] strains are essential to study their origin and evolutionary patterns. To date, all the 11 genes of only two P[19] strains, RVA/Human-wt/IND/RMC321/1990/G9P[19] and RVA/Human-wt/IND/mani-97/2006/G9P[19], have been analyzed, providing evidence for their porcine origin. In the present study, the whole genomes of the first reported human P[19] strains, RVA/Human-tc/THA/Mc323/1989/G9P[19] and RVA/Human-tc/THA/Mc345/1989/G9P[19], were analyzed. Strains Mc323 and Mc345 exhibited a G9-P[19]-I5-R1-C1-M1-A8-N1-T1-E1-H1 genotype constellation. With the exception of the NSP5 gene, both the strains were closely related to each other. Most of the genes of Mc323 (VP2-4, VP6-7, NSP1-4 genes) and Mc345 (VP2-4, VP6-7 and NSP1-5 genes) appeared to be of porcine origin, whilst the exact origin of VP1 and NSP5 genes of Mc323 and VP1 gene of Mc345 could not be ascertained. Therefore, strains Mc323 and Mc345 were found to have a porcine RVA genetic backbone, and are likely of porcine origin. Taken together, our observations corroborated the hypothesis that P[19] strains might be derived from porcine RVAs, providing important insights into the origin of P[19] strains, and on interspecies transmission of RVAs.
A 组轮状病毒（RVA）P[19]是 RVA VP4 基因的罕见 P 基因型，迄今为止仅在人类和猪中报道过。对 P[19]株的全基因组分析对于研究其起源和进化模式至关重要。迄今为止，仅对两种 P[19]株，即 RVA/Human-wt/IND/RMC321/1990/G9P[19]和 RVA/Human-wt/IND/mani-97/2006/G9P[19]的 11 个基因进行了全基因组分析，为其猪源提供了证据。在本研究中，对首次报道的人类 P[19]株，即 RVA/Human-tc/THA/Mc323/1989/G9P[19]和 RVA/Human-tc/THA/Mc345/1989/G9P[19]的全基因组进行了分析。株 Mc323 和 Mc345 表现出 G9-P[19]-I5-R1-C1-M1-A8-N1-T1-E1-H1 基因型组成。除 NSP5 基因外，两株之间的关系非常密切。Mc323 的大多数基因（VP2-4、VP6-7、NSP1-4 基因）和 Mc345（VP2-4、VP6-7 和 NSP1-5 基因）似乎源自猪，而 Mc323 的 VP1 和 NSP5 基因以及 Mc345 的 VP1 基因的确切来源尚无法确定。因此，发现株 Mc323 和 Mc345 具有猪源 RVA 遗传基础，可能源自猪。综上所述，我们的观察结果证实了 P[19]株可能源自猪源 RVAs 的假设，为 P[19]株的起源以及 RVAs 的种间传播提供了重要的见解。
