Center for Diagnostics and Vaccine Development, Centers for Disease Control, Taipei, Taiwan.
Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary.
Sci Rep. 2017 Jan 18;7:40731. doi: 10.1038/srep40731.
Molecular epidemiologic studies collecting information of the spatiotemporal distribution of rotavirus VP7 (G) and VP4 (P) genotypes have shown evidence for the increasing global importance of genotype G9 rotaviruses in humans and pigs. Sequence comparison of the VP7 gene of G9 strains identified different lineages to prevail in the respective host species although some of these lineages appear to be shared among heterologous hosts providing evidence of interspecies transmission events. The majority of these events indicates the pig-to-human spillover, although a reverse route of transmission cannot be excluded either. In this study, new variants of G9 rotaviruses were identified in two children with diarrhea and numerous pigs in Taiwan. Whole genome sequence and phylogenetic analyses of selected strains showed close genetic relationship among porcine and human strains suggesting zoonotic origin of Taiwanese human G9 strains detected in 2014-2015. Although the identified human G9P[19] and G9P[13] rotaviruses represented minority strains, the repeated detection of porcine-like rotavirus strains in Taiwanese children over time justifies the continuation of synchronized strain surveillance in humans and domestic animals.
分子流行病学研究收集轮状病毒 VP7(G)和 VP4(P)基因型的时空分布信息，表明 G9 基因型轮状病毒在人类和猪中的全球重要性日益增加。对 G9 株 VP7 基因的序列比较表明，不同的谱系在各自的宿主物种中流行，尽管其中一些谱系似乎在异源宿主中共享，这为种间传播事件提供了证据。这些事件中的大多数表明了猪向人的溢出，但也不能排除相反的传播途径。在这项研究中，在台湾腹泻的两名儿童和大量猪中发现了新的 G9 轮状病毒变异株。选择的毒株的全基因组序列和系统发育分析表明，猪与人株之间具有密切的遗传关系，提示 2014-2015 年在台湾检测到的人类 G9 株具有动物源性。尽管鉴定的人类 G9P[19]和 G9P[13]轮状病毒代表少数株，但台湾儿童中猪样轮状病毒株的反复检测证明了在人类和家畜中进行同步株监测的合理性。
