Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Curr Opin Chem Biol. 2012 Apr;16(1-2):206-13. doi: 10.1016/j.cbpa.2011.12.011. Epub 2012 Jan 7.
Here we review recent developments and trends in sample preparation, pre-fractionation, chromatography and mass spectrometry contributing towards the ultra-sensitive global analysis of proteins. Highly sensitive MS-based proteomics is not only beneficiary for the proteome analysis of single cells, an aim which is getting into reach, but also clearly relevant for the analysis of (a) subcellular organelles, (b) specific low-abundant cell-types such as adult stem cells, and (c) smaller but more homogeneous cell populations sorted or dissected from (diseased) tissue.
在这里，我们回顾了近年来在样品制备、预分级、色谱和质谱方面的最新进展和趋势，这些进展和趋势有助于实现对蛋白质的超灵敏全局分析。基于 MS 的高灵敏度蛋白质组学不仅有益于单细胞的蛋白质组分析（这一目标正在实现），而且显然与（a）亚细胞细胞器、（b）特定的低丰度细胞类型（如成人干细胞）以及（c）从（患病）组织中分离或分离出来的更小但更同质的细胞群体的分析有关。
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