细胞器和大型细胞结构的蛋白质组学

Proteomics of organelles and large cellular structures.

作者信息

Yates John R, Gilchrist Annalyn, Howell Kathryn E, Bergeron John J M

机构信息

Department of Cell Biology, 10550 North Torrey Pines Road, The Scripps Research Institute, La Jolla, California 92037, USA.

出版信息

Nat Rev Mol Cell Biol. 2005 Sep;6(9):702-14. doi: 10.1038/nrm1711.

DOI:10.1038/nrm1711

PMID:16231421

Abstract

The mass-spectrometry-based identification of proteins has created opportunities for the study of organelles, transport intermediates and large subcellular structures. Traditional cell-biology techniques are used to enrich these structures for proteomics analyses, and such analyses provide insights into the biology and functions of these structures. Here, we review the state-of-the-art proteomics techniques for the analysis of subcellular structures and discuss the biological insights that have been derived from such studies.

摘要

基于质谱的蛋白质鉴定为细胞器、运输中间体和大型亚细胞结构的研究创造了机会。传统的细胞生物学技术用于富集这些结构以进行蛋白质组学分析，此类分析为这些结构的生物学和功能提供了深入见解。在此，我们综述了用于分析亚细胞结构的最新蛋白质组学技术，并讨论了从此类研究中获得的生物学见解。