Art Unit, Drouot Laboratory, Paris, France.
Reprod Biomed Online. 2012 Feb;24(2):211-8. doi: 10.1016/j.rbmo.2011.10.006. Epub 2011 Oct 22.
The utility of sperm DNA testing remains controversial. However, it may be helpful in couples with unexplained failures of multiple assisted reproductive techniques and/or recurrent abortions. This study analysed 10,400 spermatozoa of 26 patients for sperm-head morphology with high-magnification microscopy, DNA fragmentation and sperm chromatin decondensation. A significant negative correlation was demonstrated between sperm-parameters and abnormal sperm-head morphology as assessed by high magnification (score 0 according to this study's classification): concentration (r=-0.41; P=0.03), motility (r=-0.42; P=0.03), morphology (r=-0.63; P=0.0008). No correlation was found with DNA fragmentation. However, the sperm chromatin-decondensation rate of score-0 spermatozoa was twice as high as the controls (19.5% versus 10.1%; P<0.0001). This observation suggests that score-0 spermatozoa should not be selected for intracytoplasmic sperm injection.
精子 DNA 检测的实用性仍然存在争议。然而，在多次辅助生殖技术失败且/或反复流产的夫妇中，它可能会有所帮助。本研究使用高倍显微镜分析了 26 名患者的 10400 个精子头形态、DNA 碎片化和精子染色质去浓缩。高倍镜下评估的精子参数与异常精子头形态之间存在显著负相关（根据本研究的分类，评分 0）：浓度（r=-0.41；P=0.03）、活力（r=-0.42；P=0.03）、形态（r=-0.63；P=0.0008）。与 DNA 碎片化无相关性。然而，评分 0 的精子的染色质去浓缩率是对照组的两倍（19.5%比 10.1%；P<0.0001）。这一观察结果表明，不应选择评分 0 的精子进行胞浆内单精子注射。
