Department of Chemical and Biological Engineering, University of Colorado at Boulder, UCB 424, Boulder, CO 80309, USA.
Metab Eng. 2012 May;14(3):205-11. doi: 10.1016/j.ymben.2011.12.007. Epub 2011 Dec 29.
The advancement of synthetic biology is thanks, in large part, to continuing improvements in DNA synthesis. The expansion of synthetic biology into the realm of metabolic engineering has shifted the focus from simply making novel synthetic biological parts to answering the question of how we employ these biological parts to construct genomes that ultimately give rise to useful phenotypes. Much like protein engineering, the answer to this will be arrived at following the combination of rational design and evolutionary approaches. This review will highlight some of the new DNA synthesis-enabled search methods and discuss the application of such methods to the creation of synthetic gene networks and genomes.
合成生物学的发展在很大程度上要归功于 DNA 合成技术的不断改进。随着合成生物学向代谢工程领域的扩展，人们的研究重点已经从简单地制造新型合成生物部件转移到了如何利用这些生物部件构建最终产生有用表型的基因组这一问题上。与蛋白质工程类似，这个问题的答案将是通过合理设计和进化方法的结合来得出的。本文将重点介绍一些新的 DNA 合成技术支持的搜索方法，并讨论这些方法在合成基因网络和基因组创建中的应用。
