Lin Jeng-Feng, Wu Semon, Huang Shiang-Suo, Lu Bo-Yi, Lin Su-Man, Tsai Shen-Kou
Division of Cardiology, Buddhist Tzu Chi General Hospital, Taiwan, Republic of China.
Chin J Physiol. 2011 Dec 31;54(6):406-12. doi: 10.4077/CJP.2011.AMM097.
Our prior study had shown that resveratrol was a potent cardioprotective agent in rats with myocardial infarction (MI). In this study, we further evaluated the mechanism of cardioprotection of resveratrol by proteomic analysis. After permanent ligation of the left anterior descending artery under isoflurane anesthesia, surviving rats were randomly allocated to three groups and treated with resveratrol at 1 mg/kg/day (MI/R group), or vehicles (sham group and MI group) once daily for four weeks. In proteomic analysis, the MI group showed decreased expression of adenylate kinase 1 (AK1) and mitochondrial NADP⁺-dependent isocitrate dehydrogenase (IDPm) after MI compared with the sham group. These variations were reversed by resveratrol in the MI/R group. Validation with Western blot and immunohistochemical analyses showed similar trends in protein expression profiling. Our studies suggest that the beneficial effects of resveratrol on ventricular modeling may be due to increased expression of AK1 and IDPm, which have been known to increase myocardial energetic efficiency and reduce reactive oxygen species-mediated damage, respectively.
我们之前的研究表明，白藜芦醇对心肌梗死（MI）大鼠是一种有效的心脏保护剂。在本研究中，我们通过蛋白质组学分析进一步评估了白藜芦醇的心脏保护机制。在异氟烷麻醉下永久结扎左前降支动脉后，将存活的大鼠随机分为三组，分别用1 mg/kg/天的白藜芦醇处理（MI/R组），或用赋形剂处理（假手术组和MI组），每天一次，持续四周。在蛋白质组学分析中，与假手术组相比，MI组在心肌梗死后腺苷酸激酶1（AK1）和线粒体NADP⁺依赖性异柠檬酸脱氢酶（IDPm）的表达降低。白藜芦醇在MI/R组中逆转了这些变化。蛋白质免疫印迹和免疫组织化学分析验证显示，蛋白质表达谱具有相似趋势。我们的研究表明，白藜芦醇对心室重塑的有益作用可能是由于AK1和IDPm表达增加，已知它们分别可提高心肌能量效率和减少活性氧介导的损伤。
