Department of Environmental Health Sciences, School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA 70112, USA.
Biomarkers. 2012 Mar;17(2):150-9. doi: 10.3109/1354750X.2011.645166. Epub 2012 Jan 10.
Toluene diisocyanates (2,4-TDI and 2,6-TDI) are important intermediates in the chemical industry. Among the main damages after low levels of TDI exposure are lung sensitization and asthma. It is therefore necessary to have sensitive and specific methods to monitor isocyanate exposure of workers. Urinary metabolites or protein adducts have been used as biomarkers in workers exposed to TDI. However, with these methods it was not possible to determine if the biomarkers result from exposure to TDI or to the corresponding toluene diamines (TDA). This work presents a new procedure for the determination of isocyanate-specific albumin adducts. Isotope dilution mass spectrometry was used to measure the adducts in albumin present in workers exposed to TDI. 2,4-TDI and 2,6-TDI formed adducts with lysine: N(ϵ)-[({3-amino-4-methylphenyl}amino)carbonyl]-lysine, N(ϵ)-[({5-amino-2-methylphenyl}amino)carbonyl]-lysine, and N(ϵ)- [({3-amino-2-methylphenyl}amino)carbonyl]-lysine. In future studies, this new method can be applied to measure TDI-exposures in workers.
甲苯二异氰酸酯（2,4-TDI 和 2,6-TDI）是化学工业中的重要中间体。在 TDI 低水平暴露后的主要损害中，有肺部致敏和哮喘。因此，有必要使用敏感和特异的方法来监测工人的异氰酸酯暴露。尿代谢物或蛋白质加合物已被用作接触 TDI 的工人的生物标志物。然而，使用这些方法无法确定生物标志物是源自 TDI 暴露还是源自相应的甲苯二胺（TDA）。本工作提出了一种用于测定异氰酸酯特异性白蛋白加合物的新方法。同位素稀释质谱法用于测量暴露于 TDI 的工人白蛋白中存在的加合物。2,4-TDI 和 2,6-TDI 与赖氨酸形成加合物：N(ε)-[({3-氨基-4-甲基苯基}氨基)羰基]-赖氨酸、N(ε)-[({5-氨基-2-甲基苯基}氨基)羰基]-赖氨酸和 N(ε)-[({3-氨基-2-甲基苯基}氨基)羰基]-赖氨酸。在未来的研究中，这种新方法可用于测量工人的 TDI 暴露。
