Mangas A, Yajeya J, González N, Ruiz I, Geffard M, Coveñas R
Gemacbio - Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT) - University of Salamanca.
Eur J Histochem. 2016 Dec 2;60(4):2709. doi: 10.4081/ejh.2016.2709.
Using an immunohistochemical technique, we have studied the distribution of 3-OH-anthranilic acid (3-HAA) in the rat brain. Our study was carried out in control animals and in rats in which a stroke model (single transient middle cerebral artery occlusion) was performed. A monoclonal antibody directed against 3-HAA was also developed. 3-HAA was exclusively observed in the infarcted regions (ipsilateral striatum/cerebral cortex), 2, 5 and 21 days after the induction of stroke. In control rats and in the contralateral side of the stroke animals, no immunoreactivity for 3-HAA was visualized. Under pathological conditions (from early phases of stroke), we reported for the first time the presence of 3-HAA in the mammalian brain. By double immunohistochemistry, the coexistence of 3-HAA and GFAP was observed in astrocytes. The distribution of 3-HAA matched perfectly with the infarcted regions. Our findings suggest that, in stroke, 3-HAA could be involved in the tissue damage observed in the infarcted regions, since it is well known that 3-HAA exerts cytotoxic effects.
我们运用免疫组织化学技术研究了3-羟基邻氨基苯甲酸（3-HAA）在大鼠脑中的分布。我们的研究在对照动物以及进行了中风模型（单次短暂大脑中动脉闭塞）的大鼠中开展。还制备了一种针对3-HAA的单克隆抗体。在中风诱导后2天、5天和21天，仅在梗死区域（同侧纹状体/大脑皮层）观察到3-HAA。在对照大鼠以及中风动物的对侧，未发现3-HAA的免疫反应性。在病理条件下（从中风早期阶段开始），我们首次报道了哺乳动物脑中存在3-HAA。通过双重免疫组织化学，在星形胶质细胞中观察到3-HAA与胶质纤维酸性蛋白（GFAP）共存。3-HAA的分布与梗死区域完全匹配。我们的研究结果表明，在中风中，3-HAA可能参与了梗死区域观察到的组织损伤，因为众所周知3-HAA具有细胞毒性作用。
