Grochans Elzbieta, Grzywacz Anna, Małecka Iwona, Samochowiec Agnieszka, Karakiewicz Beata, Samochowiec Jerzy
Samodzielna Pracownia Propedeutyki Pielegniarstwa PUM w Szczecinie.
Psychiatr Pol. 2011 May-Jun;45(3):325-35.
Alcohol abuse is a complex multifactorial disease. It is believed that alcohol dependence is mostly caused by genetic (40-50%) and environmental (50-60%) factors.
The aim of this study was to assess the relation between the alcohol dependence syndrome (ADS) and the polymorphism of the selected genes (GRIK3, 5HTT, ANKK1, ADH4).
The study was conducted in the Department and Clinic of Psychiatry, the Pomeranian Medical University in Szczecin in the years 2006-2008. It involved 100 patients hospitalised with Alcohol Dependence Syndrome (ADS). DNA polymorphisms were detected using the polymerase chain reaction (PCR). Statistical calculations were done with SPSS version 9.0. The chi-square test was applied to calculate the differences in the frequency of allelic forms.
Comparison between the patients with ADS and the patients from the control group demonstrated statistically significant association of ADH4 (rs1800759) with the alcohol dependence syndrome. Both the A/A genotype and the A allele were more common in patients with ADS. Also, analysis of the association of the ANKK1 gene revealed statistically significant differences (p = 0.004) between the ADS group and the control group. No statistically significant differences considering other associations were found.
Our results suggest that the analysed polymorphisms ofANKK1 and ADH4 can play an important part in the pathogenesis of alcohol abuse. The greater study group would increase the statistical power of the study and help to isolate homogeneous sub-groups of patients.
酒精滥用是一种复杂的多因素疾病。人们认为酒精依赖主要由遗传因素（40 - 50%）和环境因素（50 - 60%）引起。
本研究的目的是评估酒精依赖综合征（ADS）与所选基因（GRIK3、5HTT、ANKK1、ADH4）多态性之间的关系。
该研究于2006 - 2008年在什切青波美拉尼亚医科大学精神病学系及诊所进行。研究涉及100例因酒精依赖综合征（ADS）住院的患者。使用聚合酶链反应（PCR）检测DNA多态性。采用SPSS 9.0版进行统计计算。应用卡方检验计算等位基因形式频率的差异。
ADS患者与对照组患者之间的比较表明，ADH4（rs1800759）与酒精依赖综合征存在统计学上的显著关联。A/A基因型和A等位基因在ADS患者中更为常见。此外，ANKK1基因关联分析显示ADS组与对照组之间存在统计学显著差异（p = 0.004）。未发现其他关联存在统计学显著差异。
我们的结果表明，ANKK1和ADH4的分析多态性可能在酒精滥用的发病机制中起重要作用。更大的研究组将提高研究的统计效力，并有助于分离出患者的同质亚组。
