Departamento de Toxicología Ambiental, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, San Luis Potosí, Mexico.
Immunopharmacol Immunotoxicol. 2012 Aug;34(4):627-32. doi: 10.3109/08923973.2011.648265. Epub 2012 Jan 10.
The objective of this study was evaluate the effect of coplanar polychlorinated biphenyls (PCB) 118-congener (PCB like-dioxin) and noncoplanar PCB 153-congener (PCB no like-dioxin) on differentiation of humans T-CD4+ lymphocytes into Th1 or Th2 subpopulations. Peripheral blood mononuclear cells (PBMC) were isolated from healthy volunteers (aged 25-30 years); T-CD4+ lymphocytes were separated from PBMC. Then, the differentiation of T-CD4+ cells into Th1 or Th2 subpopulation was performed and the intracellular cytokines analyses were assessed. No effect on IFNγ (produced by Th1 cells) production was observed when the cells were treated with both PCBs congeners. However, the PCB 118-congener induced an increase of IL-4-producing T-CD4 cells (produced by Th2 cells), PCB153 not exerted any effect on IL-4 production. The clinical significance of our data is uncertain, therefore, more studies are necessary in order to elucidate the effects generated in exposed human individual.
本研究的目的是评估平面多氯联苯 (PCB) 118 同系物（类二噁英 PCB）和非平面多氯联苯 153 同系物（非类二噁英 PCB）对人 T-CD4+淋巴细胞向 Th1 或 Th2 亚群分化的影响。从健康志愿者（年龄 25-30 岁）中分离外周血单核细胞 (PBMC)；从 PBMC 中分离 T-CD4+淋巴细胞。然后，将 T-CD4+细胞分化为 Th1 或 Th2 亚群，并评估细胞内细胞因子分析。当用两种 PCB 同系物处理细胞时，IFNγ（由 Th1 细胞产生）的产生没有受到影响。然而，PCB 118 同系物诱导产生 IL-4 的 T-CD4 细胞（由 Th2 细胞产生）增加，而 PCB153 对 IL-4 的产生没有任何影响。我们数据的临床意义尚不确定，因此，需要进行更多的研究以阐明在暴露的个体中产生的影响。
