Key Laboratory of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, P R China.
Domest Anim Endocrinol. 2012 May;42(4):203-9. doi: 10.1016/j.domaniend.2011.12.003. Epub 2011 Dec 31.
The aim of the present study was to investigate the effect of cysteamine on growth performance of preweaning piglets and gastric expression of ghrelin mRNA in vivo and in vitro. Twelve litters of newborn piglets were allocated randomly to control and treatment groups. From 15 d of age, piglets in the control group were fed basal creep diet, whereas the treatment group received basal diet supplemented with 120 mg cysteamine per kg of diet until weaning on 35 d of age. Body weight gain, creep feed consumption, and diarrhea rates were recorded, and gastric mucosal tissues were collected for quantifying mRNA expression. To evaluate the direct effect of cysteamine on gastric ghrelin expression, primary cultures of gastric mucosal cells isolated from 35-d-old piglets were exposed to cysteamine for 20 h at 0, 1, 10, and 100 μg/mL, respectively. Dietary cysteamine increased (P < 0.05) average daily creep feed consumption and BW gain in preweaning pigs, which was accompanied by reduction in diarrhea rates. At 35 d of age, piglets treated with cysteamine showed increased (P < 0.05) ghrelin and gastrin and decreased (P < 0.05) somatostatin mRNA expression in gastric mucosa. Moreover, dietary cysteamine treatment increased serum concentration of gastrin (P < 0.05). In vitro, cysteamine significantly increased ghrelin mRNA expression in gastric mucosal cells at the concentration of 10 μg/mL. In conclusion, dietary cysteamine is effective in improving the growth performance and health condition of preweaning piglets, which is associated with its stimulatory effects on gastric ghrelin mRNA expression both in vivo and in vitro.
本研究旨在探讨半胱胺对新生仔猪生长性能和胃ghrelin mRNA 体内和体外表达的影响。将 12 窝新生仔猪随机分为对照组和处理组。从 15 日龄开始，对照组仔猪饲喂基础开食料，而处理组仔猪则在基础日粮中添加 120mg/kg 的半胱胺，直至 35 日龄断奶。记录体重增加、开食料采食量和腹泻率，并采集胃黏膜组织用于定量测定 mRNA 表达。为了评估半胱胺对胃 ghrelin 表达的直接影响，从 35 日龄仔猪分离出胃黏膜细胞进行原代培养，分别用 0、1、10 和 100μg/ml 的半胱胺处理 20 小时。日粮半胱胺增加了（P<0.05）断奶前仔猪的平均日开食料采食量和 BW 增加，同时降低了腹泻率。在 35 日龄时，用半胱胺处理的仔猪胃黏膜中 ghrelin 和胃泌素 mRNA 表达增加（P<0.05），而生长抑素 mRNA 表达降低（P<0.05）。此外，日粮半胱胺处理增加了血清胃泌素浓度（P<0.05）。在体外，半胱胺在 10μg/ml 的浓度下显著增加了胃黏膜细胞中 ghrelin mRNA 的表达。总之，日粮半胱胺能有效改善断奶前仔猪的生长性能和健康状况，这与其在体内和体外对胃 ghrelin mRNA 表达的刺激作用有关。
