Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, Glasgow G4 0RE, UK.
J Am Chem Soc. 2012 Jan 18;134(2):758-61. doi: 10.1021/ja2077922. Epub 2011 Dec 30.
Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,4-dinitrophenol locally in predetermined regions in response to directed irradiation with UV light via a local photolysis system. This also provides a proof of principle for the general temporally and spatially controlled release of bioactive molecules, pharmacophores, or toxins to mitochondria with tissue, cell, or mitochondrion specificity.
已使用光活化探针实现了单个线粒体或细胞内小簇线粒体的去极化。该探针通过烷基三苯基膦亲脂阳离子靶向线粒体基质，并在局部用光解系统通过定向紫外光照射在预定区域内局部释放质子载体 2,4-二硝基苯酚。这也为具有组织、细胞或线粒体特异性的生物活性分子、药效团或毒素的一般时间和空间控制释放提供了原理证明。
