Institute of Biochemistry, Swiss Federal Institute of Technology Zurich (ETHZ), Schafmattstr. 18, CH-8093 Zurich, Switzerland.
Trends Cell Biol. 2012 Mar;22(3):133-40. doi: 10.1016/j.tcb.2011.11.007. Epub 2012 Jan 10.
The endosomal sorting complex required for transport (ESCRT)-III machinery contributes to membrane deformation and scission in cytokinesis, intraluminal vesicle formation, autophagy and virus budding. Recombinant ESCRT-III subunits polymerize in vitro into filaments, tubes, sheets or rings, and ESCRT-III-dependent filaments have been observed in cells at virus bud necks and at the cytokinetic abscission site. These observations have inspired speculation about how ESCRT-III could mediate constriction and fission of membrane necks. Based on the polymer structures observed in vitro and in vivo, we discuss models for ESCRT-III function and outline how emerging technologies could be used to test these models.
内体分选复合物需要运输 (ESCRT)-III 机械有助于细胞质分裂、胞内小泡形成、自噬和病毒出芽过程中的膜变形和分裂。重组 ESCRT-III 亚基在体外聚合成长丝、管、片或环，并且在病毒芽颈和胞质分裂分离部位的细胞中观察到 ESCRT-III 依赖性丝。这些观察结果激发了关于 ESCRT-III 如何介导膜颈的收缩和分裂的推测。基于体外和体内观察到的聚合物结构，我们讨论了 ESCRT-III 功能的模型，并概述了新兴技术如何用于测试这些模型。
