Rosen N R, DiFino S, Nelson D A
Cancer. 1979 Jun;43(6):2405-9. doi: 10.1002/1097-0142(197906)43:6<2405::aid-cncr2820430635>3.0.co;2-7.
A 76-year-old woman with acute leukemia responded incompletely to prednisone and vincristine. Cytochemistry of the blast cells demonstrated only focal alpha-naphthyl acetate and alpha-naphthyl butyrate esterase activity and focal coarse granular beta-glucuronidase activity, a pattern usually associated with acute lymphocytic leukemia. Electron microscopy demonstrated primitive cells with features usually associated with promonocyte differentiation including prominent parallel arrays of microfilaments and nucleoli of the nucleolonema type. In Wright-stained films, blasts contained oval, pale-blue, yellow tinged cytoplasmic inclusions about 2 to 4 micrometers in diameter which did not stain with any of the cytochemical methods employed. With electron microscopy, these inclusions were nonmembrane bound structures, varying from amorphous electron dense stippling to irregularly arranged short segments of filaments to prominent parallel arrays of filaments. We propose that the inclusions may have arisen from the parallel arrays of microfilaments and are nonspecific.
一名76岁的急性白血病女性患者对泼尼松和长春新碱治疗反应不完全。原始细胞的细胞化学检查仅显示局灶性α-萘乙酸酯酶和α-萘丁酸酯酶活性以及局灶性粗大颗粒β-葡萄糖醛酸酶活性，这种模式通常与急性淋巴细胞白血病相关。电子显微镜检查显示原始细胞具有通常与原单核细胞分化相关的特征，包括微丝的突出平行排列和核仁样核仁。在瑞氏染色片中，原始细胞含有直径约2至4微米的椭圆形、淡蓝色、带黄色的胞质内含物，在所采用的任何细胞化学方法中均不着色。通过电子显微镜检查，这些内含物是非膜结合结构，从无定形电子致密点彩到不规则排列的短丝段再到突出的平行丝束不等。我们认为这些内含物可能源自微丝的平行排列且是非特异性的。
