Mechanical Engineering, Chemical Engineering, University of Washington, Seattle, Washington 98195-2600, United States.
Environ Sci Technol. 2012 Feb 7;46(3):1869-76. doi: 10.1021/es203661k. Epub 2012 Jan 30.
Lipid bilayers are biomembranes common to cellular life and constitute a continuous barrier between cells and their environment. Understanding the interaction of nanoparticles with lipid bilayers is an important step toward predicting subsequent biological effects. In this study, we assessed the affinity of functionalized gold nanoparticles (Au NPs) with sizes from 5 to 100 nm to lipid bilayers by determining the Au NP distribution between aqueous electrolytes and lipid bilayers. The Au NP distribution to lipid bilayers reached an apparent steady state in 24 h with smaller Au NPs distributing onto lipid bilayers more rapidly than larger ones. Au NPs distributed to lipid bilayers to a larger extent at lower pH. Tannic acid-functionalized Au NPs exhibited greater distribution to lipid bilayers than polyvinylpyrrolidone-functionalized Au NPs of the same size. Across the various Au NP sizes, we measure the lipid bilayer-water distribution coefficient (K(lipw) = C(lip)/C(w)) as 450 L/kg lipid, which is independent of dosimetric units. This work suggests that the nanoparticle-cell membrane interaction is dependent on solution chemistry and nanoparticle surface functionality. The K(lipw) value may be used to predict the affinity of spherical Au NPs across a certain size range toward lipid membranes.
脂质双层是细胞生命中常见的生物膜，构成了细胞与其环境之间的连续屏障。了解纳米颗粒与脂质双层的相互作用是预测随后生物效应的重要步骤。在这项研究中，我们通过确定金纳米颗粒（Au NPs）在水相电解质和脂质双层之间的分布，评估了 5 至 100nm 尺寸的功能化 Au NPs 与脂质双层的亲和力。较小的 Au NPs 比较大的 Au NPs 更快地分布到脂质双层上，Au NP 分布到脂质双层在 24 小时内达到明显的稳态。Au NPs 在较低 pH 值下更大量地分布到脂质双层上。单宁酸功能化的 Au NPs 比相同尺寸的聚乙烯吡咯烷酮功能化的 Au NPs 更多地分布到脂质双层上。在各种 Au NP 尺寸下，我们测量了脂质双层-水分配系数（K（lipw）= C（lip）/C（w））为 450 L/kg 脂质，与剂量单位无关。这项工作表明，纳米颗粒-细胞膜相互作用取决于溶液化学和纳米颗粒表面功能。K（lipw）值可用于预测一定尺寸范围内球形 Au NPs 对脂质膜的亲和力。
