College of Science, Jiangxi Agricultural University, Nanchang, PR China.
Anal Chim Acta. 2012 Feb 17;715:99-104. doi: 10.1016/j.aca.2011.12.011. Epub 2011 Dec 17.
In this paper, for the first time, Cu nanoparticles (CuNPs) were prepared by seed-mediated growth method with Au nanoparticles (AuNPs) playing the role of seeds. Carbon nanotubes (CNTs) and AuNPs were first dropped on the surface of glassy carbon (GC) electrode, and then the electrode was immersed into growth solution that contained CuSO(4) and hydrazine. CuNPs were successfully grown on the surface of the CNTs. The modified electrode showed a very high electrochemical activity for electrocatalytic oxidation of glucose in alkaline medium, which was utilized as the basis of the fabrication of a nonenzymatic biosensor for electrochemical detection of glucose. The biosensor can be applied to the quantification of glucose with a linear range covering from 1.0×10(-7) to 5×10(-3)M and a low detection limit of 3×10(-8)M. Furthermore, the experiment results also showed that the biosensor exhibited good reproducibility and long-term stability, as well as high selectivity with no interference from other oxidable species.
本文首次采用金纳米粒子（AuNPs）作为种子的种子介导生长法制备了铜纳米粒子（CuNPs）。首先将碳纳米管（CNTs）和 AuNPs 滴在玻璃碳（GC）电极的表面，然后将电极浸入含有 CuSO4 和联氨的生长溶液中。CuNPs 成功地生长在 CNTs 的表面上。修饰后的电极对碱性介质中葡萄糖的电催化氧化表现出非常高的电化学活性，这为电化学检测葡萄糖的非酶生物传感器的制备提供了依据。该生物传感器可用于定量检测从 1.0×10(-7)到 5×10(-3)M 的葡萄糖，检测限低至 3×10(-8)M。此外，实验结果还表明，该生物传感器具有良好的重现性和长期稳定性，以及高选择性，没有其他可氧化物质的干扰。
