Kessous A, Bibor-Hardy V, Suh M, Simard R
Cancer Res. 1979 Aug;39(8):3225-34.
Syrian hamster embryo fibroblasts were oncogenically transformed by UV-inactivated Herpes simplex type 2. Eighteen clones were isolated shortly after transformation occurred. Two clones and their tumor derivatives were studied using several techniques. The karyotype analysis revealed different chromosome patterns in the two clones and a tendency toward hypodiploidy in the tumor derivatives. All of these cell lines were shown by molecular hybridization to contain 40% of the HSV-genome in several copies. The viral DNA sequence complexity was retained in the tumor derivatives, but a decrease in the copy number was observed. Viral RNA's were detected by in situ hybridization in all the lines that were tested. Viral antigens could be observed in these transformed cells by immunofluorescence. Finally, polypeptide analysis showed three differences between normal and transformed cells.
叙利亚仓鼠胚胎成纤维细胞被紫外线灭活的2型单纯疱疹病毒致癌转化。转化发生后不久分离出18个克隆。使用几种技术对两个克隆及其肿瘤衍生物进行了研究。核型分析显示两个克隆中的染色体模式不同，且肿瘤衍生物有亚二倍体倾向。通过分子杂交表明，所有这些细胞系均含有几份占HSV基因组40%的序列。肿瘤衍生物中病毒DNA序列的复杂性得以保留，但拷贝数有所减少。通过原位杂交在所有测试的细胞系中均检测到病毒RNA。通过免疫荧光可在这些转化细胞中观察到病毒抗原。最后，多肽分析显示正常细胞和转化细胞之间存在三个差异。
