McDermott E W, Barron E T, Smyth P P, O'Higgins N J
Department of Surgery, University College, Dublin, Ireland.
Br J Surg. 1990 Oct;77(10):1179-82. doi: 10.1002/bjs.1800771029.
Malignant breast tissue is characterized by morphological and metabolic changes when compared with normal breast tissue. In this study, the cytochemical measurement of glucose-6-phosphate dehydrogenase (G6PD) activity was used to detect abnormal metabolism in breast tissue and to determine whether abnormal metabolic activity precedes morphological change during human breast carcinogenesis. Normal and benign breast tissue, morphologically normal tissue from cancer-containing breasts, and malignant breast tissue were studied. In malignant tissue, mean(s.e.m.) G6PD activity was significantly increased when compared with normal and benign tissue (9.69(2.3) versus 27.02(1.7) mean integrated extinction (MIE) x 100, P less than 0.01). G6PD activity was increased in morphologically normal tissue from cancer-containing breasts when compared with normal and benign breast tissue from breasts with no known cancer (27.02(1.7) versus 18.42(2.6) MIE x 100, P less than 0.05). These findings suggest that metabolic abnormalities precede morphological changes in breast carcinogenesis. Abnormal metabolism can be detected widely within a cancer-containing breast. The detection of such abnormality may prove helpful in identifying patients at high risk of developing breast cancer.
与正常乳腺组织相比，恶性乳腺组织具有形态和代谢变化的特征。在本研究中，采用细胞化学方法测定葡萄糖-6-磷酸脱氢酶（G6PD）活性，以检测乳腺组织中的异常代谢，并确定在人类乳腺癌发生过程中异常代谢活性是否先于形态学改变。对正常和良性乳腺组织、来自含癌乳房的形态学正常组织以及恶性乳腺组织进行了研究。与正常和良性组织相比，恶性组织中的平均（标准误）G6PD活性显著增加（平均积分消光（MIE）×100，分别为9.69（2.3）和27.02（1.7），P＜0.01）。与无已知癌症乳房的正常和良性乳腺组织相比，来自含癌乳房的形态学正常组织中的G6PD活性增加（MIE×100，分别为27.02（1.7）和18.42（2.6），P＜0.05）。这些发现表明，在乳腺癌发生过程中，代谢异常先于形态学改变。在含癌乳房内可广泛检测到异常代谢。检测到这种异常可能有助于识别患乳腺癌风险高的患者。
