Laboratory of Veterinary Clinical Pathology, Department of Veterinary Medicine, Kagoshima University, Korimoto 1-21-24, Kagoshima 890-0065, Japan.
Res Vet Sci. 2012 Oct;93(2):892-7. doi: 10.1016/j.rvsc.2011.12.017. Epub 2012 Jan 13.
The present study investigated whether renal cyclooxygenase (COX) induction is associated with the severity of chronic kidney disease (CKD) in dogs and cats. The collected kidneys were examined histopathologically and immunohistochemically. The immunoreactivities of COX-1 and COX-2 were evaluated quantitatively, and the correlations to the plasma creatinine concentrations, glomerular size, glomerulosclerosis, interstitial fibrosis, and interstitial cell infiltration were evaluated statistically. Immunoreactivities for COX-1 were heterogeneously observed in the medullary distal tubules and collecting ducts; no correlations with the severity of renal damage were detected. Immunoreactivities for COX-2 were heterogeneously observed in the macula densa (MD) regions. In dogs, the percentage of COX-2-positive MD was significantly correlated with the glomerular size. In cats, glomeruli with COX-2-positive MD had significantly higher sclerosis scores than those with COX-2-negative MD. In conclusion, renal COX-2 is induced in canine and feline CKD, especially in relation to the glomerular changes.
本研究旨在探讨犬猫慢性肾脏病（CKD）的严重程度是否与肾脏环氧化酶（COX）的诱导有关。收集的肾脏进行了组织病理学和免疫组织化学检查。定量评估了 COX-1 和 COX-2 的免疫反应性，并对其与血浆肌酐浓度、肾小球大小、肾小球硬化、间质纤维化和间质细胞浸润的相关性进行了统计学评估。COX-1 的免疫反应性在髓质远端肾小管和集合管中呈异质性观察；未发现与肾脏损伤严重程度相关。COX-2 的免疫反应性在致密斑（MD）区域呈异质性观察。在犬中，COX-2 阳性 MD 的百分比与肾小球大小显著相关。在猫中，COX-2 阳性 MD 的肾小球硬化评分明显高于 COX-2 阴性 MD 的肾小球。总之，犬猫 CKD 中肾脏 COX-2 被诱导，特别是与肾小球变化有关。
